Transcript: Mouse NM_001312867.1

Mus musculus eukaryotic translation initiation factor 4H (Eif4h), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Eif4h (22384)
Length:
2443
CDS:
111..797

Additional Resources:

NCBI RefSeq record:
NM_001312867.1
NBCI Gene record:
Eif4h (22384)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001312867.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000216321 GGGTTCTGCTATGTAGAATTT pLKO.1 357 CDS 100% 13.200 9.240 N Eif4h n/a
2 TRCN0000275601 GAGTGCATGTCAGCTGTTAAC pLKO_005 926 3UTR 100% 10.800 7.560 N EIF4H n/a
3 TRCN0000177947 GCTGTTAACAAGTGGTTTCTA pLKO.1 938 3UTR 100% 5.625 3.938 N Eif4h n/a
4 TRCN0000178145 GCTTCATCCTTTGCTTTCTTT pLKO.1 1103 3UTR 100% 5.625 3.938 N Eif4h n/a
5 TRCN0000217543 CTACACAGCTTACGTAGGAAA pLKO.1 233 CDS 100% 4.950 3.465 N Eif4h n/a
6 TRCN0000182303 GACTTCAGAGAACCCACAGAA pLKO.1 636 CDS 100% 4.950 3.465 N Eif4h n/a
7 TRCN0000200399 GTGGTTCAGAAGGAGCAAGAA pLKO.1 774 CDS 100% 4.950 3.465 N Eif4h n/a
8 TRCN0000177896 CTATCTTTAAGGATCTCAGCA pLKO.1 289 CDS 100% 2.640 1.848 N Eif4h n/a
9 TRCN0000178272 GAGACAAAGACACAGACAAAT pLKO.1 331 CDS 100% 13.200 7.920 N Eif4h n/a
10 TRCN0000181966 CAGAGACAAAGACACAGACAA pLKO.1 329 CDS 100% 4.950 2.970 N Eif4h n/a
11 TRCN0000151553 GCTTCTGAGATAAGAACCATT pLKO.1 2158 3UTR 100% 4.950 2.970 N EIF4H n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001312867.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01775 pDONR223 100% 90.7% 99.1% None (many diffs) n/a
2 ccsbBroad304_01775 pLX_304 0% 90.7% 99.1% V5 (many diffs) n/a
3 TRCN0000465612 TACCCCGGGAGATTTTAATAAGAC pLX_317 45% 90.7% 99.1% V5 (many diffs) n/a
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