Construct: ORF ccsbBroad304_01781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004317.1_s304c1, BRDN0000404293
- Derived from:
- ccsbBroadEn_01781
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WNT7B (7477)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01781
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7477 | WNT7B | Wnt family member 7B | NM_058238.3 | 100% | 100% | |
| 2 | human | 7477 | WNT7B | Wnt family member 7B | XM_011530366.1 | 95.8% | 93.2% | (many diffs) |
| 3 | mouse | 22422 | Wnt7b | wingless-type MMTV integrat... | NM_009528.3 | 91.4% | 99.1% | (many diffs) |
| 4 | mouse | 22422 | Wnt7b | wingless-type MMTV integrat... | NM_001163634.1 | 86.7% | 92.3% | (many diffs) |
| 5 | mouse | 22422 | Wnt7b | wingless-type MMTV integrat... | NM_001163633.1 | 73.1% | 79.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca cagaaacttt cgcaagtgga ttttctacgt gtttctctgc tttggcgtcc 121 tgtacgtgaa gctcggagca ctgtcatccg tggtggccct gggagccaac atcatctgca 181 acaagattcc tggcctagcc ccgcggcagc gtgccatctg ccagagtcgg cccgatgcca 241 tcattgtgat tggggagggg gcgcagatgg gcatcaacga gtgccagtac cagttccgct 301 tcggacgctg gaactgctct gccctcggcg agaagaccgt cttcgggcaa gagctccgag 361 tagggagccg tgaggctgcc ttcacgtacg ccatcaccgc ggctggcgtg gcgcacgccg 421 tcaccgctgc ctgcagccaa gggaacctga gcaactgcgg ctgcgaccgc gagaagcagg 481 gctactacaa ccaagccgag ggctggaagt ggggcggctg ctcggccgac gtgcgttacg 541 gcatcgactt ctcccggcgc ttcgtggacg ctcgggagat caagaagaac gcgcggcgcc 601 tcatgaacct gcataacaat gaggccggca ggaaggttct agaggaccgg atgcagctgg 661 agtgcaagtg ccacggcgtg tctggctcct gcaccaccaa aacctgctgg accacgctgc 721 ccaagttccg agaggtgggc cacctgctga aggagaagta caacgcggcc gtgcaggtgg 781 aggtggtgcg ggccagccgt ctgcggcagc ccaccttcct gcgcatcaaa cagctgcgca 841 gctatcagaa gcccatggag acagacctgg tgtacattga gaagtcgccc aactactgcg 901 aggaggacgc ggccacgggc agcgtgggca cgcagggccg tctctgcaac cgcacgtcgc 961 ccggcgcgga cggctgtgac accatgtgct gcggccgagg ctacaacacc caccagtaca 1021 ccaaggtgtg gcagtgcaac tgcaaattcc actggtgctg cttcgtcaag tgcaacacct 1081 gcagcgagcg caccgaggtc ttcacctgca agtgcccaac tttcttgtac aaagtggttg 1141 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1201 accggtggcg cgttaagtcg acaatcaacc tctggatta