Construct: ORF ccsbBroad304_01784
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014926.1_s304c1, BRDN0000401060
- Derived from:
- ccsbBroadEn_01784
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XPO1 (7514)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01784
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7514 | XPO1 | exportin 1 | NM_003400.4 | 100% | 100% | |
| 2 | human | 7514 | XPO1 | exportin 1 | XM_006712094.3 | 100% | 100% | |
| 3 | human | 7514 | XPO1 | exportin 1 | XM_011533097.1 | 100% | 100% | |
| 4 | human | 7514 | XPO1 | exportin 1 | XM_024453125.1 | 100% | 100% | |
| 5 | human | 7514 | XPO1 | exportin 1 | XM_024453126.1 | 100% | 100% | |
| 6 | human | 7514 | XPO1 | exportin 1 | XM_005264544.2 | 98.5% | 98.5% | 361_362ins45 |
| 7 | human | 7514 | XPO1 | exportin 1 | XM_011533098.2 | 95.7% | 95.7% | 1887_1888ins135 |
| 8 | human | 7514 | XPO1 | exportin 1 | XM_011533099.3 | 93.5% | 92.9% | (many diffs) |
| 9 | human | 7514 | XPO1 | exportin 1 | XM_005264546.2 | 87.7% | 87.7% | 0_1ins393 |
| 10 | human | 7514 | XPO1 | exportin 1 | XM_024453127.1 | 87.7% | 87.7% | 0_1ins393 |
| 11 | mouse | 103573 | Xpo1 | exportin 1 | NM_001035226.1 | 91.5% | 98% | (many diffs) |
| 12 | mouse | 103573 | Xpo1 | exportin 1 | NM_134014.3 | 91.5% | 98% | (many diffs) |
| 13 | mouse | 103573 | Xpo1 | exportin 1 | XM_006514430.2 | 91.5% | 98% | (many diffs) |
| 14 | mouse | 103573 | Xpo1 | exportin 1 | XM_017314208.1 | 85.4% | 90.9% | (many diffs) |
| 15 | mouse | 103573 | Xpo1 | exportin 1 | XM_017314209.1 | 79.9% | 85.8% | (many diffs) |
| 16 | mouse | 103573 | Xpo1 | exportin 1 | XM_017314210.1 | 79.9% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3279
- ORF length:
- 3213
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc agcaattatg acaatgttag cagaccatgc agctcgtcag ctgcttgatt 121 tcagccaaaa actggatatc aacttattag ataatgtggt gaattgctta taccatggag 181 aaggagccca gcaaagaatg gctcaagaag tactgacaca tttaaaggag catcctgatg 241 cttggacaag agtcgacaca attttggaat tttctcagaa tatgaatacg aaatactatg 301 gactacaaat tttggaaaat gtgataaaaa caaggtggaa gattcttcca aggaaccagt 361 gcgaaggaat aaaaaaatac gttgttggcc tcattatcaa gacgtcatct gacccaactt 421 gtgtagagaa agaaaaggtg tatatcggaa aattaaatat gatccttgtt cagatactga 481 aacaagaatg gcccaaacat tggccaactt ttatcagtga tattgttgga gcaagtagga 541 ccagcgaaag tctctgtcaa aataatatgg tgattcttaa actcttgagt gaagaagtat 601 ttgatttctc tagtggacag ataacccaag tcaaatctaa gcatttaaaa gacagcatgt 661 gcaatgaatt ctcacagata tttcaactgt gtcagtttgt aatggaaaat tctcaaaatg 721 ctccacttgt acatgcaacc ttggaaacat tgctcagatt tctgaactgg attcccctgg 781 gatatatttt tgagaccaaa ttaatcagca cattgattta taagttcctg aatgttccaa 841 tgtttcgaaa tgtctctctg aagtgcctca ctgagattgc tggtgtgagt gtaagccaat 901 atgaagaaca atttgtaaca ctatttactc tgacaatgat gcaactaaag cagatgcttc 961 ctttaaatac caatattcga cttgcgtact caaatggaaa agatgatgaa cagaacttca 1021 ttcaaaatct cagtttgttt ctctgcacct ttcttaagga acatgatcaa cttatagaaa 1081 aaagattaaa tctcagggaa actcttatgg aggcccttca ttatatgttg ttggtatctg 1141 aagtagaaga aactgaaatc tttaaaattt gtcttgaata ctggaatcat ttggctgctg 1201 aactctatag agagagtcca ttctctacat ctgcctctcc gttgctttct ggaagtcaac 1261 attttgatgt tcctcccagg agacagctat atttgcccat gttattcaag gtccgtttat 1321 taatggttag tcgaatggct aaaccagagg aagtattggt tgtagagaat gatcaaggag 1381 aagttgtgag agaattcatg aaggatacag attccataaa tttgtataag aatatgaggg 1441 aaacattggt ttatcttact catctggatt atgtagatac agaaagaata atgacagaga 1501 agcttcacaa tcaagtgaat ggtacagagt ggtcatggaa aaatttgaat acattgtgtt 1561 gggcaatagg ctccattagt ggagcaatgc atgaagagga cgaaaaacga tttcttgtta 1621 ctgttataaa ggatctatta ggattatgtg aacagaaaag aggcaaagat aataaagcta 1681 ttattgcatc aaatatcatg tacatagtag gtcaataccc acgttttttg agagctcact 1741 ggaaatttct gaagactgta gttaacaagc tgttcgaatt catgcatgag acccatgatg 1801 gagtccagga tatggcttgt gatactttca ttaaaatagc ccaaaaatgc cgcaggcatt 1861 tcgttcaggt tcaggttgga gaagtgatgc catttattga tgaaattttg aacaacatta 1921 acactattat ttgtgatctt cagcctcaac aggttcatac gttttatgaa gctgtggggt 1981 acatgattgg tgcacaaaca gatcaaacag tacaagaaca cttgatagaa aagtacatgt 2041 tactccctaa tcaagtgtgg gatagtataa tccagcaggc aaccaaaaat gtggatatac 2101 tgaaagatcc tgaaacagtc aagcagcttg gtagcatttt gaaaacaaat gtgagagcct 2161 gcaaagctgt tggacacccc tttgtaattc agcttggaag aatttattta gatatgctta 2221 atgtatacaa gtgcctcagt gaaaatattt ctgcagctat ccaagctaat ggtgaaatgg 2281 ttacaaagca accattgatt agaagtatgc gaactgtaaa aagggaaact ttaaagttaa 2341 tatctggttg ggtgagccga tccaatgatc cacagatggt cgctgaaaat tttgttcccc 2401 ctctgttgga tgcagttctc attgattatc agagaaatgt cccagctgct agagaaccag 2461 aagtgcttag tactatggcc ataattgtca acaagttagg gggacatata acagctgaaa 2521 tacctcaaat atttgatgct gtttttgaat gcacattgaa tatgataaat aaggactttg 2581 aagaatatcc tgaacataga acgaactttt tcttactact tcaggctgtc aattctcatt 2641 gtttcccagc attccttgct attccaccta cacagtttaa acttgttttg gattccatca 2701 tttgggcttt caaacatact atgaggaatg tcgcagatac gggcttacag atacttttta 2761 cactcttaca aaatgttgca caagaagaag ctgcagctca gagtttttat caaacttatt 2821 tttgtgatat tctccagcat atcttttctg ttgtgacaga cacttcacat actgctggtt 2881 taacaatgca tgcatcaatt cttgcatata tgtttaattt ggttgaagaa ggaaaaataa 2941 gtacatcatt aaatcctgga aatccagtta acaaccaaat ctttcttcag gaatatgtgg 3001 ctaatctcct taagtcggcc ttccctcacc tacaagatgc tcaagtaaag ctctttgtga 3061 cagggctttt cagcttaaat caagatattc ctgctttcaa ggaacattta agagatttcc 3121 tagttcaaat aaaggaattt gcaggtgaag acacttctga tttgtttttg gaagagagag 3181 aaatagccct acggcaggct gatgaagaga aacataaacg tcaaatgtct gtccctggca 3241 tctttaatcc acatgagatt ccagaagaaa tgtgtgatta cccaacattc ttgtacaaag 3301 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 3361 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta