Construct: ORF ccsbBroad304_01850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003512.1_s304c1, BRDN0000398677
- Derived from:
- ccsbBroadEn_01850
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEM1 (7979)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01850
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7979 | SEM1 | SEM1 26S proteasome complex... | NM_006304.1 | 100% | 100% | |
| 2 | human | 7979 | SEM1 | SEM1 26S proteasome complex... | NR_163951.1 | 6.7% | (many diffs) | |
| 3 | human | 7979 | SEM1 | SEM1 26S proteasome complex... | NR_163952.1 | 6.6% | (many diffs) | |
| 4 | human | 7979 | SEM1 | SEM1 26S proteasome complex... | NR_163953.1 | 6.6% | (many diffs) | |
| 5 | human | 7979 | SEM1 | SEM1 26S proteasome complex... | NR_163950.1 | 6.4% | (many diffs) | |
| 6 | mouse | 20422 | Sem1 | SEM1, 26S proteasome comple... | NM_009169.2 | 88.5% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 276
- ORF length:
- 210
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc agagaaaaag cagccggtag acttaggtct gttagaggaa gacgacgagt 121 ttgaagagtt ccctgccgaa gactgggctg gcttagatga agatgaagat gcacatgtct 181 gggaggataa ttgggatgat gacaatgtag aggatgactt ctctaatcag ttacgagctg 241 aactagagaa acatggttat aagatggaga cttcataccc aactttcttg tacaaagtgg 301 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 361 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta