Transcript: Human NM_006304.1

Homo sapiens SEM1 26S proteasome complex subunit (SEM1), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-07-14
Taxon:
Homo sapiens (human)
Gene:
SEM1 (7979)
Length:
509
CDS:
129..341

Additional Resources:

NCBI RefSeq record:
NM_006304.1
NBCI Gene record:
SEM1 (7979)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006304.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000083695 GCTGAACTAGAGAAACATGGT pLKO.1 300 CDS 100% 2.640 3.696 N SEM1 n/a
2 TRCN0000291695 GCTGAACTAGAGAAACATGGT pLKO_005 300 CDS 100% 2.640 3.696 N SEM1 n/a
3 TRCN0000293073 CACATGTCTGGGAGGATAATT pLKO_005 235 CDS 100% 15.000 10.500 N Sem1 n/a
4 TRCN0000083694 GAACTAGAGAAACATGGTTAT pLKO.1 303 CDS 100% 10.800 7.560 N SEM1 n/a
5 TRCN0000083693 CCAGAAGAAGTGTTGAAGTAA pLKO.1 345 3UTR 100% 5.625 3.938 N SEM1 n/a
6 TRCN0000291629 CCAGAAGAAGTGTTGAAGTAA pLKO_005 345 3UTR 100% 5.625 3.938 N SEM1 n/a
7 TRCN0000191721 GATAATTGGGATGATGACAAT pLKO.1 249 CDS 100% 4.950 3.465 N Sem1 n/a
8 TRCN0000298123 GATAATTGGGATGATGACAAT pLKO_005 249 CDS 100% 4.950 3.465 N Sem1 n/a
9 TRCN0000083697 GCTGGCTTAGATGAAGATGAA pLKO.1 210 CDS 100% 4.950 3.465 N SEM1 n/a
10 TRCN0000192907 GCTGGCTTAGATGAAGATGAA pLKO.1 210 CDS 100% 4.950 3.465 N Sem1 n/a
11 TRCN0000291694 GCTGGCTTAGATGAAGATGAA pLKO_005 210 CDS 100% 4.950 3.465 N SEM1 n/a
12 TRCN0000293063 GCTGGCTTAGATGAAGATGAA pLKO_005 210 CDS 100% 4.950 3.465 N Sem1 n/a
13 TRCN0000083696 GTAGAGGATGACTTCTCTAAT pLKO.1 270 CDS 100% 1.320 0.924 N SEM1 n/a
14 TRCN0000291631 GTAGAGGATGACTTCTCTAAT pLKO_005 270 CDS 100% 1.320 0.924 N SEM1 n/a
15 TRCN0000200669 GATGAAGATGAAGATGCACAT pLKO.1 219 CDS 100% 4.050 2.430 N Sem1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006304.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01850 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01850 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000474393 GAACTTTTCCATACTTAAGAAATT pLX_317 100% 100% 100% V5 n/a
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