Construct: ORF ccsbBroad304_01870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005360.1_s304c1, BRDN0000409554
- Derived from:
- ccsbBroadEn_01870
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHAF1B (8208)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01870
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8208 | CHAF1B | chromatin assembly factor 1... | NM_005441.3 | 100% | 100% | |
| 2 | human | 8208 | CHAF1B | chromatin assembly factor 1... | XM_017028477.1 | 100% | 100% | |
| 3 | human | 8208 | CHAF1B | chromatin assembly factor 1... | XM_017028478.1 | 100% | 100% | |
| 4 | human | 8208 | CHAF1B | chromatin assembly factor 1... | XM_011529755.2 | 66.5% | 65.7% | 0_1ins464;15_16ins97 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1743
- ORF length:
- 1677
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa agtcatcact tgtgaaatag cctggcacaa caaggagccc gtgtacagcc 121 tggacttcca gcatgggacg gctgggagga tccacagact ggcgtctgcc ggcgtggaca 181 ccaatgtcag gatctggaag gtagaaaagg gaccagatgg aaaagccatc gtggaatttt 241 tgtccaatct tgctcgtcat accaaagccg tcaatgttgt gcgtttttct ccaactgggg 301 aaattttagc atcgggagga gatgatgctg tcatcctatt gtggaaggtg aatgataaca 361 aggagccgga gcagatcgct tttcaggatg aggacgaggc ccagctgaac aaggagaact 421 ggacggttgt gaagactctg cggggccact tagaagatgt gtatgatatt tgctgggcaa 481 ctgatgggaa tttaatggct tctgcctctg tggataacac agccatcata tgggatgtca 541 gcaaaggaca aaagatatca atttttaatg aacataaaag ttatgtccaa ggagtaacct 601 gggacccttt gggtcaatat gttgctactc tgagctgtga cagggtgctg cgagtataca 661 gtatacagaa gaagcgtgtg gctttcaatg tttcgaagat gctgtctgga ataggggctg 721 aaggagaggc aagaagctac cggatgtttc acgacgacag catgaagtct ttcttccgta 781 gactgagttt cactcccgac ggatctttgc ttctcacgcc agctggatgt gtggaatctg 841 gtgaaaatgt aatgaatacc acttatgttt tctccaggaa gaatcttaaa aggcccatcg 901 ctcatcttcc atgtcctgga aaagccactc ttgctgttcg ctgctgtccg gtctactttg 961 aactgaggcc agtggtggaa acaggtgtgg agctgatgag tctgccctac cgcctggtgt 1021 ttgctgtggc ctcggaggat tccgtgcttc tgtatgacac ccagcagtcc ttcccttttg 1081 gttacgtgtc taatatacat taccacaccc tcagtgacat ttcatggtcc agcgatggtg 1141 ccttcctggc catttcttcc acggacggtt actgctcatt tgtgacattt gagaaagatg 1201 aacttggaat tcctttgaaa gagaagccag ttttgaacat gagaactcct gatacagcaa 1261 agaaaaccaa gagtcagaca catcgagggt cttcgccagg acccagaccg gtagagggaa 1321 cccctgccag cagaacccaa gaccccagca gccccggcac gactccccct caggccagac 1381 aggccccagc cccaacagtc atcagggacc ctccctccat cactcctgct gtcaaaagcc 1441 ccttgccggg gccttcggag gagaagaccc tgcagcccag tagtcaaaac acaaaagccc 1501 acccatcccg gagggtcact ctgaacacac tgcaagcctg gagcaagaca acaccccgga 1561 gaataaactt aacaccctta aagacggaca ctccaccaag ttctgtacca accagtgtga 1621 tttccacccc ttctacagaa gaaattcagt cagagacgcc tggagacgct cagggcagtc 1681 ccccagagct aaagcggccc agactcgatg aaaacaaagg aggcacggaa agtctggacc 1741 cttgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1801 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1861 tctggatta