Construct: ORF ccsbBroad304_01949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015291.1_s304c1, BRDN0000401615
- Derived from:
- ccsbBroadEn_01949
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMK1 (8536)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01949
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | NM_003656.5 | 100% | 100% | |
| 2 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | XM_005265516.2 | 89.5% | 84.7% | 1030_1136del;1218_1239del |
| 3 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | XM_017007354.1 | 88.1% | 88.1% | 83_84ins132 |
| 4 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | XM_024453796.1 | 83.2% | 83.2% | 0_1ins186 |
| 5 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | XM_005265517.3 | 78.9% | 74% | 83_84ins132;898_1004del;1086_1107del |
| 6 | human | 8536 | CAMK1 | calcium/calmodulin dependen... | XR_940505.2 | 68.9% | 1_139del;771_772ins113;1137_1333del | |
| 7 | mouse | 52163 | Camk1 | calcium/calmodulin-dependen... | NM_133926.2 | 89.8% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1179
- ORF length:
- 1110
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgggggca gtggaaggcc ccaggtggaa gcaggcggag gacattagag 121 acatctacga cttccgagat gttctgggca cgggggcctt ctcggaggtg atcctggcag 181 aagataagag gacgcagaag ctggtggcca tcaaatgcat tgccaaggag gccctggagg 241 gcaaggaagg cagcatggag aatgagattg ctgtcctgca caagatcaag caccccaaca 301 ttgtagccct ggatgacatc tatgagagtg ggggccacct ctacctcatc atgcagctgg 361 tgtcgggtgg ggagctcttt gaccgtattg tggaaaaagg cttctacacg gagcgggacg 421 ccagccgcct catcttccag gtgctggatg ctgtgaaata cctgcatgac ctgggcattg 481 tacaccggga tctcaagcca gagaatctgc tgtactacag cctggatgaa gactccaaaa 541 tcatgatctc cgactttggc ctctccaaga tggaggaccc gggcagtgtg ctctccaccg 601 cctgtggaac tccgggatac gtggcccctg aagtcctggc ccagaagccc tacagcaagg 661 ctgtggattg ctggtccata ggtgtcatcg cctacatctt gctctgcggt taccctccct 721 tctatgacga gaatgatgcc aaactctttg aacagatttt gaaggccgag tacgagtttg 781 actctcctta ctgggacgac atctctgact ctgccaaaga tttcatccgg cacttgatgg 841 agaaggaccc agagaaaaga ttcacctgtg agcaggcctt gcagcaccca tggattgcag 901 gagatacagc tctagataag aatatccacc agtcggtgag tgagcagatc aagaagaact 961 ttgccaagag caagtggaag caagccttca atgccacggc tgtggtgcgg cacatgagga 1021 aactgcagct gggcaccagc caggaggggc aggggcagac ggcgagccat ggggagctgc 1081 tgacaccagt ggctgggggg ccggcagctg gctgttgctg tcgagactgc tgcgtggagc 1141 cgggcacaga actgtccccc acactgcccc accagctctt gccaactttc ttgtacaaag 1201 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1261 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta