Construct: ORF ccsbBroad304_01958
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001654.1_s304c1, BRDN0000407632
- Derived from:
- ccsbBroadEn_01958
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YARS1 (8565)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01958
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8565 | YARS1 | tyrosyl-tRNA synthetase 1 | NM_003680.3 | 100% | 100% | |
| 2 | human | 8565 | YARS1 | tyrosyl-tRNA synthetase 1 | XM_011542347.2 | 60.2% | 60.2% | 0_1ins630 |
| 3 | human | 8565 | YARS1 | tyrosyl-tRNA synthetase 1 | XM_011542348.1 | 60.2% | 60.2% | 0_1ins630 |
| 4 | human | 8565 | YARS1 | tyrosyl-tRNA synthetase 1 | XM_017002651.2 | 60.2% | 60.2% | 0_1ins630 |
| 5 | mouse | 107271 | Yars | tyrosyl-tRNA synthetase | NM_134151.4 | 83.8% | 90.4% | (many diffs) |
| 6 | mouse | 107271 | Yars | tyrosyl-tRNA synthetase | XR_376292.1 | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1650
- ORF length:
- 1584
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ggacgctccc agccctgaag agaaactgca ccttatcacc cggaacctgc 121 aggaggttct gggggaagag aagctgaagg agatactgaa ggagcgggaa cttaaaattt 181 actggggaac ggcaaccacg ggcaaaccac atgtggctta ctttgtgccc atgtcaaaga 241 ttgcagactt cttaaaggca gggtgtgagg taacaattct gtttgcggac ctccacgcat 301 acctggataa catgaaagcc ccatgggaac ttctagaact ccgagtcagt tactatgaga 361 atgtgatcaa agcaatgctg gagagcattg gtgtgccctt ggagaagctc aagttcatca 421 aaggcactga ttaccagctc agcaaagagt acacactaga tgtgtacaga ctctcctccg 481 tggtcacaca gcacgattcc aagaaggctg gagctgaggt ggtaaagcag gtggagcacc 541 ctttgctgag tggcctctta taccccggac tgcaggcttt ggatgaagag tatttaaaag 601 tagatgccca atttggaggc attgatcaga gaaagatttt cacctttgca gagaagtacc 661 tccctgcact tggctattca aaacgggtcc atctgatgaa tcctatggtt ccaggattaa 721 caggcagcaa aatgagctct tcagaagagg agtccaagat tgatctcctt gatcggaagg 781 aggatgtgaa gaaaaaactg aagaaggcct tctgtgagcc aggaaatgtg gagaacaatg 841 gggttctgtc cttcatcaag catgtccttt ttccccttaa gtccgagttt gtgatcctac 901 gagatgagaa atggggtgga aacaaaacct acacagctta cgtggacctg gaaaaggact 961 ttgctgctga ggttgtacat cctggagacc tgaagaattc tgttgaagtc gcactgaaca 1021 agttgctgga tccaatccgg gaaaagttta atacccctgc cctgaaaaaa ctggccagcg 1081 ctgcctaccc agatccctca aagcagaagc caatggccaa aggccctgcc aagaattcag 1141 aaccagagga ggtcatccca tcccggctgg atatccgtgt ggggaaaatc atcactgtgg 1201 agaagcaccc agatgcagac agcctgtatg tagagaagat tgacgtgggg gaagctgaac 1261 cacggactgt ggtgagcggc ctggtacagt tcgtgcccaa ggaggaactg caggacaggc 1321 tggtagtggt gctgtgcaac ctgaaacccc agaagatgag aggagtcgag tcccaaggca 1381 tgcttctgtg tgcttctata gaagggataa accgccaggt tgaacctctg gaccctccgg 1441 caggctctgc tcctggtgag cacgtgtttg tgaagggcta tgaaaagggc caaccagatg 1501 aggagctcaa gcccaagaag aaagtcttcg agaagttgca ggctgacttc aaaatttctg 1561 aggagtgcat cgcacagtgg aagcaaacca acttcatgac caagctgggc tccatttcct 1621 gtaaatcgct gaaagggggg aacattagct acccaacttt cttgtacaaa gtggttggta 1681 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1741 ggtggcgcgt taagtcgaca atcaacctct ggatta