Transcript: Human XM_011542348.1

PREDICTED: Homo sapiens tyrosyl-tRNA synthetase 1 (YARS1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
YARS1 (8565)
Length:
2184
CDS:
486..1442

Additional Resources:

NCBI RefSeq record:
XM_011542348.1
NBCI Gene record:
YARS1 (8565)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011542348.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000045635 GCTTTGGATGAAGAGTATTTA pLKO.1 319 5UTR 100% 15.000 10.500 N YARS1 n/a
2 TRCN0000291656 GCTTTGGATGAAGAGTATTTA pLKO_005 319 5UTR 100% 15.000 10.500 N YARS1 n/a
3 TRCN0000303340 TTATGCCACAGTCCTTATAAT pLKO_005 1741 3UTR 100% 15.000 10.500 N YARS1 n/a
4 TRCN0000045636 CCCTGCACTTGGCTATTCAAA pLKO.1 452 5UTR 100% 5.625 3.938 N YARS1 n/a
5 TRCN0000174078 CCCTGCACTTGGCTATTCAAA pLKO.1 452 5UTR 100% 5.625 3.938 N YARS1 n/a
6 TRCN0000307763 CCCTGCACTTGGCTATTCAAA pLKO_005 452 5UTR 100% 5.625 3.938 N YARS1 n/a
7 TRCN0000045634 GCCAGGAAATGTGGAGAACAA pLKO.1 608 CDS 100% 4.950 3.465 N YARS1 n/a
8 TRCN0000045637 GCACTGAACAAGTTGCTGGAT pLKO.1 801 CDS 100% 2.640 1.848 N YARS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011542348.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01958 pDONR223 100% 60.2% 60.2% None 0_1ins630 n/a
2 ccsbBroad304_01958 pLX_304 0% 60.2% 60.2% V5 0_1ins630 n/a
3 TRCN0000467421 CTATCCCGCCAGCTTGCGTGCTTA pLX_317 26.1% 60.2% 60.2% V5 0_1ins630 n/a
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