Construct: ORF ccsbBroad304_01973

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000692.1_s304c1, BRDN0000410751
Derived from:
ccsbBroadEn_01973
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SKAP1 (8631)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01973
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8631 SKAP1 src kinase associated phosp... NM_001075099.2 100% 100%
2 human 8631 SKAP1 src kinase associated phosp... NM_003726.4 99.7% 99.7% 876_878delAGT
3 human 8631 SKAP1 src kinase associated phosp... XM_005257755.4 95.2% 95.2% 824_825ins51
4 human 8631 SKAP1 src kinase associated phosp... XM_024451012.1 94% 84.1% (many diffs)
5 human 8631 SKAP1 src kinase associated phosp... XM_017025257.2 93% 81% 0_1ins46;104_105ins26;804_806delAGT
6 human 8631 SKAP1 src kinase associated phosp... XM_017025258.1 74.6% 74% (many diffs)
7 human 8631 SKAP1 src kinase associated phosp... XM_017025259.1 73.5% 73.5% 0_1ins282;594_596delAGT
8 human 8631 SKAP1 src kinase associated phosp... XM_017025260.1 73.5% 73.5% 0_1ins282;594_596delAGT
9 mouse 78473 Skap1 src family associated phosp... NM_001033186.3 84.6% 80.2% (many diffs)
10 mouse 78473 Skap1 src family associated phosp... NM_001177899.1 81.6% 77.9% (many diffs)
11 mouse 78473 Skap1 src family associated phosp... XM_006534508.3 78.4% 71.1% (many diffs)
12 mouse 78473 Skap1 src family associated phosp... NM_001177898.1 75.5% 68.9% (many diffs)
13 mouse 78473 Skap1 src family associated phosp... XM_017314838.1 65.8% 58.4% (many diffs)
14 mouse 78473 Skap1 src family associated phosp... XM_017314839.1 62.9% 56.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1140
ORF length:
1074
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca ggccgccgcc ctccctgagg agatccgttg gctcctggaa gatgctgaag
121agtttctggc agaaggtttg cggaatgaga acctcagcgc tgttgcaagg gatcacagag
181accatattct acggggcttt cagcaaatca aagccaggta ctattgggat tttcagcccc
241aagggggaga cattggacag gacagctctg atgataatca cagcgggact cttggcctgt
301ccctcacatc cgatgcaccc tttttgtcag attatcagga tgagggaatg gaagacatcg
361taaaaggagc tcaagaactt gataacgtaa tcaagcaagg atacttggag aagaaaagca
421aagatcatag tttctttgga tcggagtggc agaagcgatg gtgtgttgtc agcagaggtc
481tcttctacta ctatgctaat gagaagagca agcagcccaa agggaccttc ctcattaagg
541gctacggtgt acggatggcc ccccacctgc gaagagattc caagaaagaa tcctgctttg
601aactgacctc ccaggatagg cgcagctatg agtttacagc tactagtcca gcagaagcca
661gagactgggt ggatcaaata agtttcttgt taaaggatct gagctcctta accattccat
721atgaagagga tgaggaggaa gaagaaaaag aagagacata tgatgatatt gatggttttg
781actccccaag ttgtggttcc cagtgcagac ccactatctt gcctgggagt gtggggataa
841aagagcctac agaggagaaa gaagaagaag atatttatga agtcttgcca gatgaagagc
901atgatctaga agaggatgag agtggcactc gacgaaaagg agactatgcc agttactacc
961agggcctatg ggattgccat ggtgaccagc cagatgaact gtccttccaa cggggtgacc
1021tcatccgtat tctgagcaag gagtataaca tgtatggctg gtgggtggga gaactgaaca
1081gcctcgttgg gattgttcca aaggagtatc tcaccactgc ctttgaagtg gaagaaagat
1141gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1201attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1261ggatta

Download FASTA (ORF) (Full)