Construct: ORF ccsbBroad304_01986
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013860.1_s304c1, BRDN0000406431
- Derived from:
- ccsbBroadEn_01986
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF3I (8668)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01986
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8668 | EIF3I | eukaryotic translation init... | NM_003757.3 | 100% | 100% | |
| 2 | human | 442720 | EIF3IP1 | eukaryotic translation init... | NR_003024.1 | 84.3% | (many diffs) | |
| 3 | human | 8668 | EIF3I | eukaryotic translation init... | XM_024450518.1 | 59% | 46.9% | 0_1ins121;127_128ins278 |
| 4 | mouse | 54709 | Eif3i | eukaryotic translation init... | NM_018799.2 | 91.2% | 99.3% | (many diffs) |
| 5 | mouse | 75030 | 4930503E24Rik | RIKEN cDNA 4930503E24 gene;... | NR_028310.1 | 63.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gccgatccta ctgcagggcc atgagcggtc cattacgcag attaagtata 121 accgcgaagg agacctcctc tttactgtgg ccaaggaccc tatcgtcaat gtatggtact 181 ctgtgaatgg tgagaggctg ggcacctaca tgggccatac cggagctgtg tggtgtgtgg 241 acgctgactg ggacaccaag catgtcctca ctggctcagc tgacaacagc tgtcgtctct 301 gggactgtga aacaggaaag cagctggccc ttctcaagac caattcggct gtccggacct 361 gcggttttga ctttgggggc aacatcatca tgttctccac ggacaagcag atgggctacc 421 agtgctttgt gagctttttt gacctgcggg atccgagcca gattgacaac aatgagccct 481 acatgaagat cccttgcaat gactctaaaa tcaccagtgc tgtttgggga cccctggggg 541 agtgcatcat cgctggccat gagagtggag agctcaacca gtatagtgcc aagtctggag 601 aggtgttggt gaatgttaag gagcactccc ggcagatcaa cgacatccag ttatccaggg 661 acatgaccat gtttgtgacc gcgtccaagg acaacacagc caagcttttt gactccacaa 721 ctcttgaaca tcagaagact ttccggacag aacgtcctgt caactcagct gccctctccc 781 ccaactatga ccatgtggtc ctgggcggtg gtcaggaagc catggatgta accacaacct 841 ccaccaggat tggcaagttt gaggccaggt tcttccattt ggcctttgaa gaagagtttg 901 gaagagtcaa gggtcacttt ggacctatca acagtgttgc cttccatcct gatggcaaga 961 gctacagcag cggcggcgaa gatggttacg tccgtatcca ttacttcgac ccacagtact 1021 tcgaatttga gtttgaggct tacccaactt tcttgtacaa agtggttggt aagcctatcc 1081 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1141 ttaagtcgac aatcaacctc tggatta