Construct: ORF ccsbBroad304_02008
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002015.1_s304c1, BRDN0000400079
- Derived from:
- ccsbBroadEn_02008
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDS2 (8760)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02008
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8760 | CDS2 | CDP-diacylglycerol synthase 2 | NM_003818.4 | 100% | 100% | |
| 2 | human | 8760 | CDS2 | CDP-diacylglycerol synthase 2 | XM_006723660.2 | 85.7% | 71.2% | 980_999del;1182_1183ins173 |
| 3 | human | 8760 | CDS2 | CDP-diacylglycerol synthase 2 | XM_011529390.1 | 82.4% | 82.4% | 0_1ins234 |
| 4 | human | 8760 | CDS2 | CDP-diacylglycerol synthase 2 | XM_024452016.1 | 82.4% | 82.4% | 0_1ins234 |
| 5 | mouse | 110911 | Cds2 | CDP-diacylglycerol synthase... | NM_138651.7 | 88.6% | 96.1% | (many diffs) |
| 6 | mouse | 110911 | Cds2 | CDP-diacylglycerol synthase... | NM_001291039.1 | 85.6% | 92.8% | (many diffs) |
| 7 | mouse | 110911 | Cds2 | CDP-diacylglycerol synthase... | NM_001291040.1 | 25% | 27.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1401
- ORF length:
- 1335
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac agagctgagg cagagggtgg cccatgagcc ggttgcgcca cccgaggaca 121 aggagtcaga gtcagaagca aaggtagatg gagagactgc atcggacagt gagagccggg 181 cagaatccgc acccctgcca gtctctgcag atgatacccc ggaggtcctc aatagggccc 241 tttccaactt gtcttcaaga tggaagaact ggtgggtgag aggcatcctg actttggcca 301 tgattgcatt tttcttcatc atcatttacc tgggaccaat ggttttgatg ataatcgtga 361 tgtgcgttca gattaagtgt ttccatgaga taatcactat tggctacaac gtctaccact 421 catatgatct gccctggttc aggacgctca gctggtactt tctcctgtgt gtaaactatt 481 tcttctatgg tgagacagtg acggattact tcttcaccct ggtccagaga gaagagcctt 541 tgcggattct cagtaaatac caccggttca tttcctttac tctctatcta ataggattct 601 gcatgtttgt actgagtctg gtcaagaagc attatcgact gcagttctac atgtttggct 661 ggacccatgt gacattgctg attgttgtaa cacagtcaca tcttgttatc cacaacctat 721 ttgaaggaat gatctggttc attgtcccca tatcttgtgt gatctgtaat gacatcatgg 781 cctatatgtt tggctttttc tttggtcgga ccccactcat caagctgtcc ccgaagaaga 841 cctgggaagg cttcattggg ggcttctttg ctactgtggt gtttggcctt ctgctgtcct 901 atgtgatgtc cgggtacaga tgctttgtct gccctgtgga gtacaacaat gacaccaaca 961 gcttcactgt ggactgtgag ccctcggacc tgtttcgcct gcaggagtac aacattcctg 1021 gggtgatcca gtcagtcatt ggctggaaaa cggtccggat gtaccccttc cagattcaca 1081 gcatcgctct ctccaccttt gcctcgctca ttggcccctt tggaggattc ttcgcaagtg 1141 gattcaaacg agcctttaaa atcaaagact ttgccaatac cattcctggc catggaggca 1201 tcatggatcg ctttgactgc cagtatctga tggccacctt tgtcaatgta tacatcgcca 1261 gttttatcag aggccctaac ccaagcaaac tgattcagca gttcctgact ttacggccag 1321 atcagcagct ccacatcttc aacacgctgc ggtctcatct gatcgacaaa gggatgctga 1381 catccaccac agaggacgag tacccaactt tcttgtacaa agtggttggt aagcctatcc 1441 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1501 ttaagtcgac aatcaacctc tggatta