Construct: ORF ccsbBroad304_02083
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006372.1_s304c1, BRDN0000398930
- Derived from:
- ccsbBroadEn_02083
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V0D1 (9114)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02083
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9114 | ATP6V0D1 | ATPase H+ transporting V0 s... | NM_004691.5 | 100% | 100% | |
| 2 | human | 9114 | ATP6V0D1 | ATPase H+ transporting V0 s... | XM_011523444.1 | 78% | 78% | 0_1ins231 |
| 3 | human | 9114 | ATP6V0D1 | ATPase H+ transporting V0 s... | XM_011523445.1 | 78% | 78% | 0_1ins231 |
| 4 | human | 9114 | ATP6V0D1 | ATPase H+ transporting V0 s... | XM_011523446.1 | 78% | 78% | 0_1ins231 |
| 5 | mouse | 11972 | Atp6v0d1 | ATPase, H+ transporting, ly... | NM_013477.3 | 90.1% | 99.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc gttcttcccg gagctttact ttaacgtgga caatggctac ttggagggac 121 tggtgcgcgg cctgaaggcc ggggtgctca gccaggccga ctacctcaac ctggtgcagt 181 gcgagacgct agaggacttg aaactgcatc tgcagagcac tgattatggt aacttcctgg 241 ccaacgaggc atcacctctg acggtgtcag tcatcgatga ccggctcaag gagaagatgg 301 tggtggagtt ccgccacatg aggaaccatg cctatgagcc actcgccagc ttcctagact 361 tcattactta cagttacatg atcgacaacg tgatcctgct catcacaggc acgctgcacc 421 agcgctccat cgctgagctc gtgcccaagt gccacccact aggcagcttc gagcagatgg 481 aggccgtgaa cattgctcag acacctgctg agctctacaa tgccattctg gtggacacgc 541 ctcttgcggc ttttttccag gactgcattt cagagcagga ccttgacgag atgaacatcg 601 agatcatccg caacaccctc tacaaggcct acctggagtc cttctacaag ttctgcaccc 661 tactgggcgg gactacggct gatgccatgt gccccatcct ggagtttgaa gcagaccgcc 721 gcgccttcat catcaccatc aattctttcg gcacagagct gtccaaagag gaccgtgcca 781 agctctttcc acactgtggg cggctctacc ctgagggcct ggcgcagctg gctcgggctg 841 acgactatga acaggtcaag aacgtggccg attactaccc ggagtacaag ctgctcttcg 901 agggtgcagg tagcaaccct ggagacaaga cgctggagga ccgattcttt gagcacgagg 961 taaagctgaa caagttggcc ttcctgaacc agttccactt tggtgtcttc tatgccttcg 1021 tgaagctcaa ggagcaggag tgtcgcaaca tcgtgtggat cgctgaatgt atcgcccagc 1081 gccaccgcgc caaaatcgac aactacatcc ctatcttcta cccaactttc ttgtacaaag 1141 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1201 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta