Construct: ORF ccsbBroad304_02107
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006823.1_s304c1, BRDN0000407116
- Derived from:
- ccsbBroadEn_02107
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGI1 (9211)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02107
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9211 | LGI1 | leucine rich glioma inactiv... | NM_005097.4 | 99.9% | 100% | 657T>C |
| 2 | human | 9211 | LGI1 | leucine rich glioma inactiv... | NM_001308276.1 | 91.3% | 91.3% | 284_285ins144 |
| 3 | human | 9211 | LGI1 | leucine rich glioma inactiv... | NR_131777.1 | 61.9% | (many diffs) | |
| 4 | human | 9211 | LGI1 | leucine rich glioma inactiv... | NM_001308275.1 | 51.1% | 50.6% | (many diffs) |
| 5 | human | 9211 | LGI1 | leucine rich glioma inactiv... | XM_017016911.2 | 50.2% | 50.2% | 657T>C;840_841delTTinsCA;843_844ins828 |
| 6 | human | 9211 | LGI1 | leucine rich glioma inactiv... | XM_017016912.2 | 42.4% | 42% | (many diffs) |
| 7 | mouse | 56839 | Lgi1 | leucine-rich repeat LGI fam... | NM_020278.2 | 91.9% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1737
- ORF length:
- 1671
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga atcagaaaga agcaaaagga tgggaaatgc ctgcattccc ctgaaaagaa 121 ttgcttattt cctatgtctc ttatctgcgc ttttgctgac tgaggggaag aaaccagcga 181 agccaaaatg ccctgccgtg tgtacttgta ccaaagataa tgctttatgt gagaatgcca 241 gatccattcc acgcaccgtt cctcctgatg ttatctcatt atcctttgtg agatctggtt 301 ttactgaaat ctcagaaggg agttttttat tcacgccatc gctgcagctc ttgttattca 361 catcgaactc ctttgatgtg atcagtgatg atgcttttat tggtcttcca catctagagt 421 atttattcat agaaaacaac aacatcaagt caatttcaag acatactttc cggggactaa 481 agtcattaat tcacttgagc cttgcaaaca acaatctcca gacactccca aaagatattt 541 tcaaaggcct ggattcttta acaaatgtgg acctgagggg taattcattt aattgtgact 601 gtaaactgaa atggctagtg gaatggcttg gccacaccaa tgcaactgtt gaagacatct 661 actgcgaagg ccccccagaa tacaagaagc gcaaaatcaa tagtctctcc tcgaaggatt 721 tcgattgcat cattacagaa tttgcaaagt ctcaagacct gccttatcaa tcattgtcca 781 tagacacttt ttcttatttg aatgatgagt atgtagtcat cgctcagcct tttactggaa 841 aatgcatttt ccttgaatgg gaccatgtgg aaaagacctt ccggaattat gacaacatta 901 caggcacatc cactgtagta tgcaagccta tagtcattga aactcagctc tatgttattg 961 tggcccagct gtttggtggc tctcacatct ataagcgaga cagttttgca aataaattca 1021 taaaaatcca ggatattgaa attctcaaaa tccgaaaacc caatgacatt gaaacattca 1081 agattgaaaa caactggtac tttgttgttg ctgacagttc aaaagctggt tttactacca 1141 tttacaaatg gaacggaaac ggattctact cccatcaatc cttacacgcg tggtacaggg 1201 acactgatgt ggaatatcta gaaatagtca gaacacctca gacactcaga acgcctcatt 1261 taattctgtc tagtagttcc cagcgtcctg taatttatca gtggaacaaa gcaacacaat 1321 tattcactaa ccaaactgac attcctaaca tggaggatgt gtacgcagtg aagcacttct 1381 cagtgaaagg ggacgtgtac atttgcttga caagattcat tggtgattcc aaagtcatga 1441 aatggggagg ctcctcgttc caggatattc agaggatgcc atcgcgagga tccatggtgt 1501 tccagcctct tcaaataaat aattaccaat atgcaattct tggaagtgat tactccttta 1561 ctcaagtgta taactgggat gcagagaaag ccaaatttgt gaaatttcag gaattaaatg 1621 ttcaggcacc aagatcattc acacatgtgt ccattaataa gcgtaatttt ctttttgctt 1681 ccagttttaa gggaaataca cagatttaca aacatgtcat agttgactta agcgcatgcc 1741 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1801 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1861 tta