Construct: ORF ccsbBroad304_02107

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006823.1_s304c1, BRDN0000407116
Derived from:
ccsbBroadEn_02107
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LGI1 (9211)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02107
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9211 LGI1 leucine rich glioma inactiv... NM_005097.4 99.9% 100% 657T>C
2 human 9211 LGI1 leucine rich glioma inactiv... NM_001308276.1 91.3% 91.3% 284_285ins144
3 human 9211 LGI1 leucine rich glioma inactiv... NR_131777.1 61.9% (many diffs)
4 human 9211 LGI1 leucine rich glioma inactiv... NM_001308275.1 51.1% 50.6% (many diffs)
5 human 9211 LGI1 leucine rich glioma inactiv... XM_017016911.2 50.2% 50.2% 657T>C;840_841delTTinsCA;843_844ins828
6 human 9211 LGI1 leucine rich glioma inactiv... XM_017016912.2 42.4% 42% (many diffs)
7 mouse 56839 Lgi1 leucine-rich repeat LGI fam... NM_020278.2 91.9% 96.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1737
ORF length:
1671
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga atcagaaaga agcaaaagga tgggaaatgc ctgcattccc ctgaaaagaa
121ttgcttattt cctatgtctc ttatctgcgc ttttgctgac tgaggggaag aaaccagcga
181agccaaaatg ccctgccgtg tgtacttgta ccaaagataa tgctttatgt gagaatgcca
241gatccattcc acgcaccgtt cctcctgatg ttatctcatt atcctttgtg agatctggtt
301ttactgaaat ctcagaaggg agttttttat tcacgccatc gctgcagctc ttgttattca
361catcgaactc ctttgatgtg atcagtgatg atgcttttat tggtcttcca catctagagt
421atttattcat agaaaacaac aacatcaagt caatttcaag acatactttc cggggactaa
481agtcattaat tcacttgagc cttgcaaaca acaatctcca gacactccca aaagatattt
541tcaaaggcct ggattcttta acaaatgtgg acctgagggg taattcattt aattgtgact
601gtaaactgaa atggctagtg gaatggcttg gccacaccaa tgcaactgtt gaagacatct
661actgcgaagg ccccccagaa tacaagaagc gcaaaatcaa tagtctctcc tcgaaggatt
721tcgattgcat cattacagaa tttgcaaagt ctcaagacct gccttatcaa tcattgtcca
781tagacacttt ttcttatttg aatgatgagt atgtagtcat cgctcagcct tttactggaa
841aatgcatttt ccttgaatgg gaccatgtgg aaaagacctt ccggaattat gacaacatta
901caggcacatc cactgtagta tgcaagccta tagtcattga aactcagctc tatgttattg
961tggcccagct gtttggtggc tctcacatct ataagcgaga cagttttgca aataaattca
1021taaaaatcca ggatattgaa attctcaaaa tccgaaaacc caatgacatt gaaacattca
1081agattgaaaa caactggtac tttgttgttg ctgacagttc aaaagctggt tttactacca
1141tttacaaatg gaacggaaac ggattctact cccatcaatc cttacacgcg tggtacaggg
1201acactgatgt ggaatatcta gaaatagtca gaacacctca gacactcaga acgcctcatt
1261taattctgtc tagtagttcc cagcgtcctg taatttatca gtggaacaaa gcaacacaat
1321tattcactaa ccaaactgac attcctaaca tggaggatgt gtacgcagtg aagcacttct
1381cagtgaaagg ggacgtgtac atttgcttga caagattcat tggtgattcc aaagtcatga
1441aatggggagg ctcctcgttc caggatattc agaggatgcc atcgcgagga tccatggtgt
1501tccagcctct tcaaataaat aattaccaat atgcaattct tggaagtgat tactccttta
1561ctcaagtgta taactgggat gcagagaaag ccaaatttgt gaaatttcag gaattaaatg
1621ttcaggcacc aagatcattc acacatgtgt ccattaataa gcgtaatttt ctttttgctt
1681ccagttttaa gggaaataca cagatttaca aacatgtcat agttgactta agcgcatgcc
1741caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1801ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1861tta

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