Construct: ORF ccsbBroad304_02124
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010095.1_s304c1, BRDN0000406308
- Derived from:
- ccsbBroadEn_02124
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AIMP1 (9255)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02124
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9255 | AIMP1 | aminoacyl tRNA synthetase c... | NM_001142415.1 | 100% | 100% | |
| 2 | human | 9255 | AIMP1 | aminoacyl tRNA synthetase c... | NM_001142416.2 | 100% | 100% | |
| 3 | human | 9255 | AIMP1 | aminoacyl tRNA synthetase c... | NM_004757.3 | 100% | 100% | |
| 4 | human | 9255 | AIMP1 | aminoacyl tRNA synthetase c... | XM_017008835.2 | 100% | 100% | |
| 5 | human | 9255 | AIMP1 | aminoacyl tRNA synthetase c... | XM_017008836.2 | 100% | 100% | |
| 6 | mouse | 13722 | Aimp1 | aminoacyl tRNA synthetase c... | NM_007926.2 | 81.4% | 83.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1002
- ORF length:
- 936
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc aaataatgat gctgttctga agagactgga gcagaagggt gcagaggcag 121 atcaaatcat tgaatatctt aagcagcaag tttctctact taaggagaaa gcaattttgc 181 aggcaacttt gagggaagag aagaaacttc gagttgaaaa tgctaaactg aagaaagaaa 241 ttgaagaact gaaacaagag ctaattcagg cagaaattca aaatggagtg aagcaaatac 301 catttccatc tggtactcca ctgcacgcta attctatggt ttctgaaaat gtgatacagt 361 ctacagcagt aacaaccgta tcttctggta ccaaagaaca gataaaagga ggaacaggag 421 acgaaaagaa agcgaaagag aaaattgaaa agaaaggaga gaagaaggag aaaaaacagc 481 aatcaatagc tggaagtgcc gactctaagc caatagatgt ttcccgtctg gatcttcgaa 541 ttggttgcat cataactgct agaaaacacc ctgatgcaga ttctttgtat gtggaagaag 601 tagatgtcgg agaaatagcc ccaaggacag ttgtcagtgg cctggtgaat catgttcctc 661 ttgaacagat gcaaaatcgg atggtgattt tactttgtaa cctgaaacct gcaaagatga 721 ggggagtatt atctcaagca atggtcatgt gtgctagttc accagagaaa attgaaatct 781 tggctcctcc aaatgggtct gttcctggag acagaattac ttttgatgct ttcccaggag 841 agcctgacaa ggagctgaat cctaagaaga agatttggga gcagatccag cctgatcttc 901 acactaatga tgagtgtgtg gctacataca aaggagttcc ctttgaggtg aaagggaagg 961 gagtatgtag ggctcaaacc atgagcaaca gtggaatcaa atacccaact ttcttgtaca 1021 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1081 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta