Construct: ORF ccsbBroad304_02134
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001273.1_s304c1, BRDN0000402531
- Derived from:
- ccsbBroadEn_02134
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NREP (9315)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02134
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142476.1 | 100% | 100% | |
| 2 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142477.1 | 100% | 100% | |
| 3 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142478.2 | 100% | 100% | |
| 4 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142479.1 | 100% | 100% | |
| 5 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142480.1 | 100% | 100% | |
| 6 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142481.1 | 100% | 100% | |
| 7 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142482.1 | 100% | 100% | |
| 8 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142483.1 | 100% | 100% | |
| 9 | human | 9315 | NREP | neuronal regeneration relat... | NM_004772.4 | 100% | 100% | |
| 10 | human | 9315 | NREP | neuronal regeneration relat... | XM_006714733.2 | 77.7% | 77% | 1_55delinsA;58_60delTCT |
| 11 | human | 9315 | NREP | neuronal regeneration relat... | XM_006714732.1 | 72.7% | 72% | 1_73delinsA;76_78delTCT |
| 12 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142474.2 | 66.6% | 65.6% | 1_95del;98_104delGTTTCCA |
| 13 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142475.2 | 60.4% | 59.8% | 1_132delinsA;135delT |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 270
- ORF length:
- 204
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ttattaccca gaactctttg tctgggtcag tcaagaacca tttccaaaca 121 aggacatgga gggaaggctt cctaagggaa gacttcctgt cccaaaggaa gtgaaccgca 181 agaagaacga tgagacaaac gctgcctccc tgactccact gggcagcagt gaactccgct 241 ccccaagaat cagttacctc cacttttttt acccaacttt cttgtacaaa gtggttggta 301 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 361 ggtggcgcgt taagtcgaca atcaacctct ggatta