Construct: ORF ccsbBroad304_02139
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013199.1_s304c1, BRDN0000398683
- Derived from:
- ccsbBroadEn_02139
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGN3 (9324)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02139
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_138730.3 | 100% | 100% | |
| 2 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001201362.2 | 80% | 81% | (many diffs) |
| 3 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_004242.4 | 77.7% | 77.7% | 219_259del;273_297del |
| 4 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001318888.2 | 69.8% | 63.6% | (many diffs) |
| 5 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001318884.2 | 61.2% | 62% | (many diffs) |
| 6 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001318885.2 | 60.6% | 60.6% | 13_14ins51;168_208del;222_246del |
| 7 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001318886.2 | 59.3% | 60.1% | (many diffs) |
| 8 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001201363.2 | 58.4% | 59.2% | (many diffs) |
| 9 | human | 9324 | HMGN3 | high mobility group nucleos... | NM_001318887.2 | 58.4% | 59.2% | (many diffs) |
| 10 | mouse | 94353 | Hmgn3 | high mobility group nucleos... | NM_175074.2 | 91.3% | 92.2% | (many diffs) |
| 11 | mouse | 94353 | Hmgn3 | high mobility group nucleos... | NM_026122.4 | 71% | 69.6% | (many diffs) |
| 12 | mouse | 94353 | Hmgn3 | high mobility group nucleos... | XM_006511590.3 | 53.4% | 52.6% | (many diffs) |
| 13 | mouse | 94353 | Hmgn3 | high mobility group nucleos... | XM_006511591.3 | 53.4% | 52.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 297
- ORF length:
- 231
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc gaagagaaag tctccagaga atacagaggg caaagatgga tccaaagtaa 121 ctaaacagga gcccacaaga cggtctgcca gattgtcagc gaaacctgct ccaccaaaac 181 ctgaacccaa accaagaaaa acatctgcta agaaagaacc tggagcaaag attagcagag 241 gtgctaaagg gaagaaggag gaaaagcagg aagctggaaa ggaaggcaca gaaaactgcc 301 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 361 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 421 tta