Construct: ORF ccsbBroad304_02148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000065.1_s304c1, BRDN0000400296
- Derived from:
- ccsbBroadEn_02148
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC22A6 (9356)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02148
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9356 | SLC22A6 | solute carrier family 22 me... | NM_153276.3 | 100% | 100% | |
| 2 | human | 9356 | SLC22A6 | solute carrier family 22 me... | XM_017018562.2 | 99.8% | 99.8% | 920_922delAGC |
| 3 | human | 9356 | SLC22A6 | solute carrier family 22 me... | NM_004790.5 | 97.6% | 97.6% | 1563_1601del |
| 4 | human | 9356 | SLC22A6 | solute carrier family 22 me... | NM_153277.3 | 92% | 92% | 1360_1361ins132 |
| 5 | human | 9356 | SLC22A6 | solute carrier family 22 me... | NM_153278.3 | 89.8% | 89.8% | 1360_1361ins132;1431_1469del |
| 6 | mouse | 18399 | Slc22a6 | solute carrier family 22 (o... | NM_008766.3 | 84.3% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1716
- ORF length:
- 1650
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ctttaatgac ctcctgcagc aggtgggggg tgtcggccgc ttccagcaga 121 tccaggtcac cctggtggtc ctccccctgc tcctgatggc ttctcacaac accctgcaga 181 acttcactgc tgccatccct acccaccact gccgcccgcc tgccgatgcc aacctcagca 241 agaacggggg gctggaggtc tggctgcccc gggacaggca ggggcagcct gagtcctgcc 301 tccgcttcac ctccccgcag tggggactgc cctttctcaa tggcacagaa gccaatggca 361 caggggccac agagccctgc accgatggct ggatctatga caacagcacc ttcccatcta 421 ccatcgtgac tgagtgggac cttgtgtgct ctcacagggc cctacgccag ctggcccagt 481 ccttgtacat ggtgggggtg ctgctcggag ccatggtgtt cggctacctt gcagacaggc 541 taggccgccg gaaggtactc atcttgaact acctgcagac agctgtgtca gggacctgcg 601 cagccttcgc acccaacttc cccatctact gcgccttccg gctcctctcg ggcatggctc 661 tggctggcat ctccctcaac tgcatgacac tgaatgtgga gtggatgccc attcacacac 721 gggcctgcgt gggcaccttg attggctatg tctacagcct gggccagttc ctcctggctg 781 gtgtggccta cgctgtgccc cactggcgcc acctgcagct actggtctct gcgccttttt 841 ttgccttctt catctactcc tggttcttca ttgagtcggc ccgctggcac tcctcctccg 901 ggaggctgga cctcaccctg agggccctgc agagagtcgc ccggatcaat gggaagcggg 961 aagaaggagc caaattgagt atggaggtac tccgggccag tctgcagaag gagctgacca 1021 tgggcaaagg ccaggcatcg gccatggagc tgctgcgctg ccccaccctc cgccacctct 1081 tcctctgcct ctccatgctg tggtttgcca ctagctttgc atactatggg ctggtcatgg 1141 acctgcaggg ctttggagtc agcatctacc taatccaggt gatctttggt gctgtggacc 1201 tgcctgccaa gcttgtgggc ttccttgtca tcaactccct gggtcgccgg cctgcccaga 1261 tggctgcact gctgctggca ggcatctgca tcctgctcaa tggggtgata ccccaggacc 1321 agtccattgt ccgaacctct cttgctgtgc tggggaaggg ttgtctggct gcctccttca 1381 actgcatctt cctgtatact ggggaactgt atcccacaat gatccggcag acaggcatgg 1441 gaatgggcag caccatggcc cgagtgggca gcatcgtgag cccactggtg agcatgactg 1501 ccgagctcta cccctccatg cctctcttca tctacggtgc tgttcctgtg gccgccagcg 1561 ctgtcactgt cctcctgcca gagaccctgg gccagccact gccagacacg gtgcaggacc 1621 tggagagcag gaaagggaaa cagacgcgac agcaacaaga gcaccagaag tatatggtcc 1681 cactgcaggc ctcagcacaa gagaagaatg gactctgccc aactttcttg tacaaagtgg 1741 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1801 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta