Construct: ORF ccsbBroad304_02175

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001172.1_s304c1, BRDN0000404994
Derived from:
ccsbBroadEn_02175
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAGED1 (9500)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02175
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9500 MAGED1 MAGE family member D1 NM_001005332.2 100% 100%
2 human 9500 MAGED1 MAGE family member D1 NM_006986.4 100% 100%
3 human 9500 MAGED1 MAGE family member D1 XM_011530835.2 100% 100%
4 human 9500 MAGED1 MAGE family member D1 XM_017029978.1 100% 100%
5 human 9500 MAGED1 MAGE family member D1 XM_017029979.1 100% 100%
6 human 9500 MAGED1 MAGE family member D1 XM_017029980.1 100% 100%
7 human 9500 MAGED1 MAGE family member D1 NM_001005333.2 93.2% 93.2% 46_213del
8 mouse 94275 Maged1 melanoma antigen, family D, 1 NM_019791.2 86.8% 86.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2400
ORF length:
2334
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tcagaaaatg gactgtggtg cgggcctcct cggcttccag gctgaggcct
121ccgtagaaga cagcgccttg cttatgcaga ccttgatgga ggccatccag atctcagagg
181ctccacctac taaccaggcc accgcagctg ctagtcccca gagttcacag cccccaactg
241ccaatgagat ggctgacatt caggtttcag cagctgccgc taggcctaag tcagccttta
301aagtccagaa tgccaccaca aaaggcccaa atggtgtcta tgatttctct caggctcata
361atgccaagga tgtgcccaac acgcagccca aggcagcctt taagtcccaa aatgctaccc
421caaagggtcc aaatgctgcc tatgattttt cccaggcagc aaccactggt gagttagctg
481ctaacaagtc tgagatggcc ttcaaggccc agaatgccac tactaaagtg ggcccaaatg
541ccacctacaa tttctctcag tctctcaatg ccaatgacct ggccaacagc aggcctaaga
601cccctttcaa ggcttggaat gataccacta aggccccaac agctgatacc cagacccaga
661atgtaaatca ggccaaaatg gccacttccc aggctgacat agagaccgac ccaggtatct
721ctgaacctga cggtgcaact gcacagacat cagcagatgg ttcccaggct cagaatctgg
781agtcccggac aataattcgg ggcaagagga cccgcaagat taataacttg aatgttgaag
841agaacagcag tggggatcag aggcgggccc cactggctgc agggacctgg aggtctgcac
901cagttccagt gaccactcag aacccacctg gcgcaccccc caatgtgctc tggcagacgc
961cattggcttg gcagaacccc tcaggctggc aaaaccagac agccaggcag accccaccag
1021cacgtcagag ccctccagct aggcagaccc caccagcctg gcagaaccca gtcgcttggc
1081agaacccagt gatttggcca aacccagtaa tctggcagaa cccagtgatc tggccaaacc
1141ccattgtctg gcccggccct gttgtctggc cgaatccact ggcctggcag aatccacctg
1201gatggcagac tccacctgga tggcagaccc caccgggctg gcagggtcct ccagactggc
1261aaggtcctcc tgactggccg ctaccacccg actggccact gccacctgat tggccacttc
1321ccactgactg gccactacca cctgactgga tccccgctga ttggccaatt ccacctgact
1381ggcagaacct gcgcccctcg cctaacctgc gcccttctcc caactcgcgt gcctcacaga
1441acccaggtgc tgcacagccc cgagatgtgg cccttcttca ggaaagagca aataagttgg
1501tcaagtactt gatgcttaag gactacacaa aggtgcccat caagcgctca gaaatgctga
1561gagatatcat ccgtgaatac actgatgttt atccagaaat cattgaacgt gcatgctttg
1621tcctagagaa gaaatttggg attcaactga aagaaattga caaagaagaa cacctgtata
1681ttctcatcag tacccccgag tccctggctg gcatactggg aacgaccaaa gacacaccca
1741agctcggtct cctcttggtg attctgggtg tcatcttcat gaatggcaac cgtgccagtg
1801aggctgtcct ctgggaggca ctacgcaaga tgggactgcg tcctggggtg agacatcccc
1861tccttggaga tctaaggaaa cttctcacct atgagtttgt aaagcagaaa tacctggact
1921acagacgagt gcccaacagc aaccccccgg agtatgagtt cctctggggc ctccgttcct
1981accatgagac tagcaagatg aaagtgctga gattcattgc agaggttcag aaaagagacc
2041ctcgtgactg gactgcacag ttcatggagg ctgcagatga ggccttggat gctctggatg
2101ctgctgcagc tgaggccgaa gcccgggctg aagcaagaac ccgcatggga attggagatg
2161aggctgtgtc tgggccctgg agctgggatg acattgagtt tgagctgctg acctgggatg
2221aggaaggaga ttttggagat ccctggtcca gaattccatt taccttctgg gccagatacc
2281accagaatgc ccgctccaga ttccctcaga cctttgccgg tcccattatt ggtcctggtg
2341gtacagccag tgccaacttc gctgccaact ttggtgccat tggtttcttc tgggttgagt
2401gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
2461attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
2521ggatta

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