Construct: ORF ccsbBroad304_02201

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013073.1_s304c1, BRDN0000405237
Derived from:
ccsbBroadEn_02201
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CLOCK (9575)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02201
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9575 CLOCK clock circadian regulator NM_001267843.1 99.9% 100% 1764T>C
2 human 9575 CLOCK clock circadian regulator NM_004898.4 99.9% 100% 1764T>C
3 human 9575 CLOCK clock circadian regulator XM_005265787.2 99.9% 100% 1764T>C
4 human 9575 CLOCK clock circadian regulator XM_011534409.2 99.9% 100% 1764T>C
5 human 9575 CLOCK clock circadian regulator XM_011534410.2 99.9% 100% 1764T>C
6 human 9575 CLOCK clock circadian regulator XM_011534411.2 99.9% 100% 1764T>C
7 human 9575 CLOCK clock circadian regulator XM_017008854.1 99.9% 100% 1764T>C
8 human 9575 CLOCK clock circadian regulator XM_024454284.1 99.9% 100% 1764T>C
9 human 9575 CLOCK clock circadian regulator XM_017008855.1 92.9% 93% 0_1ins177;1587T>C
10 mouse 12753 Clock circadian locomotor output ... NM_001305222.1 89.2% 94.6% (many diffs)
11 mouse 12753 Clock circadian locomotor output ... XM_017320649.1 89.2% 94.6% (many diffs)
12 mouse 12753 Clock circadian locomotor output ... NM_001289826.1 89.1% 94.5% (many diffs)
13 mouse 12753 Clock circadian locomotor output ... NM_007715.6 89.1% 94.5% (many diffs)
14 mouse 12753 Clock circadian locomotor output ... XM_017320648.1 89.1% 94.5% (many diffs)
15 mouse 12753 Clock circadian locomotor output ... XM_017320651.1 85.9% 91.5% (many diffs)
16 mouse 12753 Clock circadian locomotor output ... XM_017320650.1 85.8% 91.3% (many diffs)
17 mouse 12753 Clock circadian locomotor output ... XM_011249402.1 75.5% 80.2% (many diffs)
18 mouse 12753 Clock circadian locomotor output ... XR_001784602.1 22.9% (many diffs)
19 mouse 12753 Clock circadian locomotor output ... XR_001784601.1 22.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2607
ORF length:
2538
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gttgtttacc gtaagctgta gtaaaatgag ctcgattgtt gacagagatg
121acagtagtat ttttgatggg ttggtggaag aagatgacaa ggacaaagcg aaaagagtat
181ctagaaacaa atctgaaaag aaacgtagag atcaatttaa tgttctcatt aaagaactgg
241gatccatgct tcctggtaat gctagaaaga tggacaaatc tactgttctg cagaaaagca
301ttgatttttt acgaaaacat aaagaaatca ctgcacagtc agatgctagt gaaattcgac
361aggactggaa acctacattc cttagtaatg aagagtttac acaattaatg ttagaggctc
421ttgatggttt ttttttagca atcatgacag atggaagcat aatatatgtg tctgagagtg
481taacttcatt acttgaacat ttaccatctg atcttgtgga tcaaagtata tttaatttta
541tcccagaagg ggaacattca gaggtttata aaatactctc tactcatctg ctggaaagtg
601attcattaac cccagaatat ttaaaatcaa aaaatcagtt agaattctgt tgtcacatgc
661tgcgaggaac aatagaccca aaggagccat ctacctatga atatgtaaaa tttataggaa
721atttcaaatc tttaaacagt gtatcctctt cagcacacaa tggttttgaa ggaactatac
781aacgcacaca taggccatct tatgaagata gagtttgttt tgtagctact gtcaggttag
841ctacacctca gttcatcaag gaaatgtgca ctgttgaaga acccaatgaa gagtttacat
901ctagacatag tttagaatgg aagtttctgt ttctagatca cagggcacca cccataatag
961ggtatttgcc atttgaagtt ctgggaacat caggctatga ttactatcat gtggatgacc
1021tagaaaattt ggcaaaatgt catgagcact taatgcaata tgggaaaggc aaatcatgtt
1081attataggtt cctgactaag gggcaacagt ggatttggct tcagactcat tattatatca
1141cttaccatca gtggaattca aggccagagt ttattgtttg tactcacact gtagtaagtt
1201atgcagaagt tagggctgaa agacgacgag aacttggcat tgaagagtct cttcctgaga
1261cagctgctga caaaagccaa gattctgggt cagataatcg tataaacaca gtcagtctca
1321aggaagcatt ggaaaggttt gatcacagcc caaccccttc tgcctcttct cggagttcaa
1381gaaaatcatc tcacacggcc gtctcagacc cttcctcaac accaaccaag atcccgacgg
1441atacgagcac tccacccagg cagcatttac cagctcatga gaagatggtg caaagaaggt
1501catcatttag tagtcagtcc ataaattccc agtctgttgg ttcatcatta acacagccag
1561tgatgtctca agctacaaat ttaccaattc cacaaggcat gtcccagttt cagttttcag
1621ctcaattagg agccatgcaa catctgaaag accaattgga acaacggaca cgcatgatag
1681aagcaaatat tcatcggcaa caagaagaac taagaaaaat tcaagaacaa cttcagatgg
1741tccatggtca ggggctgcag atgtttttgc aacaatcaaa tcctgggttg aattttggtt
1801ccgttcaact ttcttctgga aattcatcta acatccagca acttgcacct ataaatatgc
1861aaggccaagt tgttcctact aaccagattc aaagtggaat gaatactgga cacattggca
1921caactcagca catgATACAA CAACAGACTT TACAGAGTAC ATCAACTCAG AGTCAACAAA
1981ATGTACTGAG TGGGCACAGT CAGCAAACAT CTCTACCCAG TCAGACACAG AGCACTCTTA
2041CAGCCCCACT GTATAACACT ATGGTGATTT CTCAGCCTGC AGCCGGAAGC ATGGTCCAGA
2101TTCCATCTAG TATGCCACAA AACAGCACCC AGAGTGCTGC AGTAACTACA TTCACTCAGG
2161ACAGGCAGAT AAGATTTTCT CAAGGTCAAC AACTTGTGAC CAAATTAGTG ACTGCTCCTG
2221TAGCTTGTGG GGCAGTCATG GTACCTAGTA CTATGCTTAT GGGCCAGGTG GTGACTGCAT
2281ATCCTACTTT TGCTACACAA CAGCAACAGT CACAGACATT GTCAGTAACG CAGCAGCAGC
2341AGCAGCAGAG CTCCCAGGAG CAGCAGCTCA CTTCAGTTCA GCAACCATCT CAGGCTCAGC
2401TGACCCAGCC ACCGCAACAA TTTTTACAGA CTTCTAGGTT GCTCCATGGG AATCCCTCAA
2461CTCAACTCAT TCTCTCTGCT GCATTTCCTC TACAACAGAG CACCTTCCCT CAGTCACATC
2521ACCAGCAACA TCAGTCTCAG CAACAGCAGC AACTCAGCCG GCACAGGACT GACAGCTTGC
2581CCGACCCTTC CAAGGTTCAA CCACAGTTGC CAACTTTCTT GTACaaagtg gttggtaagc
2641ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
2701ggcgcgttaa gtcgacaatc aacctctgga tta

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