Construct: ORF ccsbBroad304_02233
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016122.1_s304c1, BRDN0000462300
- Derived from:
- ccsbBroadEn_02233
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFT140 (9742)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02233
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9742 | IFT140 | intraflagellar transport 140 | NM_014714.4 | 100% | 100% | |
| 2 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_006720990.3 | 100% | 100% | |
| 3 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_006720991.3 | 100% | 100% | |
| 4 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_017023910.1 | 100% | 100% | |
| 5 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_011522766.3 | 94.3% | 94.3% | 1522_1523ins246 |
| 6 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_011522767.2 | 77.5% | 76.7% | (many diffs) |
| 7 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_017023911.1 | 58.6% | 58.6% | 0_1ins1815 |
| 8 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_011522769.3 | 55.4% | 54.9% | (many diffs) |
| 9 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_005255725.5 | 55% | 54.7% | (many diffs) |
| 10 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_011522771.3 | 54.9% | 54.7% | (many diffs) |
| 11 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_011522772.3 | 54.8% | 54.7% | (many diffs) |
| 12 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_005255726.4 | 54.7% | 54.6% | 2398_2399insGT;2402C>G;2403_2404ins1981 |
| 13 | human | 9742 | IFT140 | intraflagellar transport 140 | XM_006720992.3 | 45.9% | 45.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4452
- ORF length:
- 4386
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaagaag 61 ttggcatggc cctctattat gaccaccaga tagaagcccc ggatgcagca gggtcaccct 121 catttatcag ctggcaccct gtccatccat tcttggcagt tgcttacatc agcacaacct 181 caacaggcag cgtggatatt tacctggagc aaggggagtg cgtgccagat acacacgtcg 241 agaggccgtt ccgggttgct tccctgtgct ggcacccgac gcggctggtg ctggctgtgg 301 gctgggagac tggagaagtg acggtgttta acaagcagga caaggagcag cacacgatgc 361 ccctgacaca cacagccgac atcaccgtgc tccgttggag ccccagtgga aactgcctgc 421 tgtctgggga caggcttggt gtcttgctct tgtggaggtt ggaccaaagg ggccgagtgc 481 aagggacgcc tctgctgaaa cacgagtatg ggaaacacct cacgcactgc atcttccggc 541 tcccccctcc tggcgaggac ctggttcagt tggcaaaggc agctgtgagc ggtgatgaga 601 aagccctgga catgtttaac tggaagaaga gcagttctgg aagtttgctg aagatggggt 661 ctcacgaggg gctgttgttc tttgtcagtc tgatggacgg gacagtgcac tatgtggatg 721 agaagggcaa gaccactcag gtggtgtccg cagacagcac gattcagatg ctgttctaca 781 tggagaagag ggaggcactg gtggtggtca cagagaacct ccggctgtcc ctgtacacgg 841 tgcctcctga gggcaaagca gaagaagtga tgaaggtcaa gctgagcggg aaaaccggcc 901 gccgggcaga catcgctttg attgaaggca gccttctcgt gatggccgtc ggggaggctg 961 ccctcagatt ctgggacata gaacgaggag agaattatat actgagtcca gatgagaagt 1021 ttggctttga gaaaggagag aatatgaact gtgtgtgtta ctgtaaagtc aaaggtcttc 1081 tggccgctgg taccgacaga gggcgagtag ccatgtggag gaaagtacca gacttcctgg 1141 gcagccccgg ggcagagggc aaggacaggt gggcccttca gacccctacc gagctccaag 1201 gaaacatcac gcaaatccag tggggttcca ggaagaacct gctggcagtg aacagcgtca 1261 tctccgtggc catcctcagc gagcgggcca tgtcgtcaca cttccaccag caagtggccg 1321 ccatgcaggt ctccccgagt ctgctgaatg tgtgcttcct gtccacgggg gtcgcacaca 1381 gcctgcgcac cgacatgcac atcagtggag tgtttgccac caaggatgct gtcgcagtct 1441 ggaacggaag gcaggtggcg atcttcgagc tttctggagc cgcgatacgg agtgcaggga 1501 ccttcttgtg tgagacgcct gtgttagcaa tgcatgaaga aaacgtttac acggtggagt 1561 caaaccgagt tcaagttcga acctggcagg ggactgtcaa acaactcctc cttttctcgg 1621 agactgaggg gaatccctgc ttcttggaca tctgtgggaa tttcctggtt gtagggacag 1681 acttggctca ctttaaaagc tttgatcttt cccgaagaga ggccaaagca cactgtagct 1741 gcaggagcct ggcggagctg gtccctgggg tggggggcat cgcttctctg cggtgcagca 1801 gcagcgggag caccatcagc atcctcccca gcaaggctga caacagccct gattccaaaa 1861 tctgcttcta cgatgttgaa atggacacag tgaccgtctt tgacttcaag actggacaaa 1921 ttgatcggag agagacgctg tcctttaatg agcaagagac taataagagc cacctctttg 1981 tggatgaggg actgaaaaat tatgttcccg tgaaccactt ctgggaccag agtgagcccc 2041 ggctgtttgt atgcgaagcc gtgcaggaga cgccgcgctc ccagcctcag tctgcaaacg 2101 ggcagcccca agatgggcgc gctggccctg cggcagatgt tttgatcctg tccttcttca 2161 tttccgaaga gcacggcttc ctgcttcatg agagcttccc ccggcctgcc acctcccaca 2221 gtctcctggg gatggaagtg ccttattact acttcacaag aaagcccgaa gaagcagaca 2281 gagaagacga ggtggagcct gggtgccacc acatccctca gatggtgtcc aggagacccc 2341 tgcgagactt tgtggggctg gaggactgcg acaaggccac ccgggacgcc atgctccact 2401 tcagcttctt tgtcaccata ggagacatgg acgaagcctt caaatccatc aagctcatca 2461 aaagtgaggc cgtctgggag aacatggcgc gcatgtgcgt gaagacccag cggctggacg 2521 tggccaaggt gtgcctgggg aacatgggcc atgcccgcgg ggcccgagcg ctgcgtgagg 2581 cggagcagga gccggagcta gaggcccgcg tggccgtgct ggccacgcag ctgggcatgc 2641 tggaggacgc cgagcagctg tacaggaagt gcaagcgcca cgacctcctg aacaagttct 2701 accaggctgc gggccggtgg caggaggccc tccaggtagc cgagcaccac gatcgcgtgc 2761 acctgcgcag cacctaccac cgctatgccg ggcacctgga ggccagcgcc gactgcagcc 2821 gggccctcag ttactacgag aagtcggaca cgcaccgctt cgaggtgccc aggatgctgt 2881 cggaggacct gccgtccctg gagctctacg tgaataaaat gaaggataag accctgtggc 2941 ggtggtgggc gcagtacctg gagagccagg gcgagatgga cgccgcgctg cactactacg 3001 agctggcccg ggaccacttc tccctggtcc gcatccactg cttccagggc aatgtccaga 3061 aggctgcgca aatagccaac gagacaggaa acctggcggc ctcctaccac ctcgcccgcc 3121 agtacgagag ccaggaggag gtcgggcagg cggtgcactt ctacacccgg gcacaggcct 3181 tcaagaatgc catccgcctg tgcaaggaga acggcctgga cgaccagctc atgaacttgg 3241 ccctgctgag ctcccccgag gacatgatcg aggcggcccg atactacgag gagaagggcg 3301 tgcagatgga cagggcggtc atgctgtacc acaaggctgg ccacttctcc aaggccctgg 3361 agctggcctt tgccacccag cagtttgtgg ccctacagct catagcagag gacctggatg 3421 agacgtcaga ccctgcgctc ctggcccgct gctccgactt cttcatcgag cacagtcagt 3481 acgagagggc ggtagagctg ctgctggctg ccaggaagta tcaggaagcc ctgcagctgt 3541 gcctggggca gaacatgagc atcaccgagg agatggcgga aaagatgacc gtggccaagg 3601 actcctcgga cctgcctgag gagtcgcggc gggagctgct ggagcagata gcagactgct 3661 gcatgcgcca gggcagctac cacctggcca ccaagaagta cacgcaggcc ggcaacaagc 3721 tgaaggccat gagggcgctg ctcaaatccg gagacacgga gaaaatcacg ttcttcgcga 3781 gcgtgtccag gcagaaggaa atctacatca tggctgctaa ctacctgcag tccctggact 3841 ggcggaagga gccggagatc atgaagaaca tcatcggctt ctacaccaag gggcgggccc 3901 tggacctcct ggctggcttt tatgacgctt gtgcccaggt ggagattgat gaataccaga 3961 actacgacaa agcccacggg gcgctgaccg aggcctacaa gtgcctggcc aaggccaagg 4021 ccaagagccc cctggaccag gagaccaggc tggcgcagct gcagagcagg atggcactgg 4081 tgaagaggtt catccaggcc cgcaggacgt acacagagga ccccaaggag tccatcaagc 4141 agtgtgagct gctcctggag gaaccagacc tggacagcac catccgcatc ggggacgtct 4201 atggcttcct ggtggagcac tacgtgcgga aggaggaata ccagacggcc tacagattcc 4261 tggaggagat gcggcggcgg cttcccttgg ccaacatgtc ctactacgtg agcccgcagg 4321 ccgtggacgc cgtgcaccgg gggctgggtc tcccactgcc acgcaccgtc cccgagcagg 4381 tccgccacaa cagcatggag gacgccaggg agctggacga ggaggtggtg gaagaggcag 4441 atgacgaccc ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 4501 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 4561 caatcaacct ctggatta