Construct: ORF ccsbBroad304_02235

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009451.1_s304c1, BRDN0000407194
Derived from:
ccsbBroadEn_02235
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STARD8 (9754)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02235
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9754 STARD8 StAR related lipid transfer... NM_001142504.3 100% 100%
2 human 9754 STARD8 StAR related lipid transfer... NM_014725.4 100% 100%
3 human 9754 STARD8 StAR related lipid transfer... XM_011531069.3 100% 100%
4 human 9754 STARD8 StAR related lipid transfer... XM_005262315.3 99.6% 99.6% 55_66del
5 human 9754 STARD8 StAR related lipid transfer... XM_011531070.2 99.6% 99.6% 55_66del
6 human 9754 STARD8 StAR related lipid transfer... NM_001142503.2 92.7% 92.7% 1_240del
7 human 9754 STARD8 StAR related lipid transfer... XM_005262314.4 92.4% 92.4% 1_240del;295_306del
8 mouse 236920 Stard8 START domain containing 8 NM_199018.2 83.2% 84.9% (many diffs)
9 mouse 236920 Stard8 START domain containing 8 XM_006528004.2 83.2% 84.9% (many diffs)
10 mouse 236920 Stard8 START domain containing 8 XM_006528007.1 83.2% 84.9% (many diffs)
11 mouse 236920 Stard8 START domain containing 8 XM_006528005.1 81.9% 83.2% (many diffs)
12 mouse 236920 Stard8 START domain containing 8 XM_006528006.1 81.9% 83.2% (many diffs)
13 mouse 236920 Stard8 START domain containing 8 XM_006528003.3 81.6% 82.2% (many diffs)
14 mouse 236920 Stard8 START domain containing 8 XM_006528002.1 81.5% 82.1% (many diffs)
15 mouse 236920 Stard8 START domain containing 8 XM_006527999.3 77.5% 79.1% (many diffs)
16 mouse 236920 Stard8 START domain containing 8 XM_006527998.1 77.3% 78.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3135
ORF length:
3069
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgac cttgaataat tgtgcctcga tgaaactgga ggttcatttt caaagcaagc
121agaatgaaga ctcagaagag gaagagcagt gtaccatcag tagccactgg gccttccagc
181aggaaagtaa gtgctggtct cctatggggt cctctgatct gttggcccca ccgagccctg
241gcctgccagc gacctcaagc tgtgagagcg tcctcaccga gcttagtgcc acctctctgc
301cagtcatcac cgtgagccta ccacccgagc cagcagactt gcccttgcca ggccgtgccc
361ccagctcgag tgaccggccc ctcctcagcc ccacccaggg ccaggagggt ccccaggaca
421aagccaagaa gcgccatcgt aaccgtagct tcctcaagca ccttgaatct ctgaggcgga
481aggaaaagag tggcagccag caagcagagc ccaagcatag tccagccacc tcagagaagg
541tctccaaagc ctcatctttc cgcagttgtc gtggcttcct ctcagctgga ttttacaggg
601ccaagaactg ggccgccacc tcagccggtg gcagtggtgc caatactcgg aaggcctggg
661aggcctggcc tgtggcctcg ttccggcatc ctcagtggac acaccggggt gattgcctgg
721tgcacgttcc tggggaccac aaaccaggca cattccctcg ctccctgtcc attgagagcc
781tgtgtcctga ggatggacac cgcctggcag actggcagcc aggtaggcgg tggggctgtg
841aggggcgccg gggctcctgt ggctcaacgg gcagccatgc cagcacgtat gacaacttgc
901ctgagctgta cccagctgag cctgtaatgg ttggggctga ggctgaagat gaagatgatg
961aggagagtgg gggcagctat gctcacctag acgacatcct ccagcacgtg tgggggctac
1021agcaacgagt agagctgtgg tctcgggcca tgtacccaga cctggggcct ggagatgagg
1081aagaggagga ggccacttca tcagtagaaa tagccacagt tgaggtcaaa tgccaagctg
1141aggctctcag ccagatggag gttccggccc atggagagtc cccagcctgg gcccaggctg
1201aagtccagcc agcagtcctg gctccggctc aggctccagc tgaggctgaa ccagtggcac
1261aggaagaggc tgaggccccg gccccagccc cggccccggc cccagcccag gacagtgagc
1321aggaggcaca ttcaggcggg gaacccacct ttgcctctag cctgtctgtg gaagaaggac
1381actccatttc tgacactgtg gcctcctcca gcgaacttga cagtagtggg aactccatga
1441atgaggctga ggctgcgggg cccctggctg gactccaggc atcaatgccc cgtgaacggc
1501gcgattcagg tgttggggcc tcacttacca gaccctgcag gaagctccgt tggcatagct
1561tccagaactc ccatcgtccc agcctcaact cagagtcgct ggagatcaac cggcagtttg
1621caggccagat caacctcctg cacaagggct cactgctgcg gcttaccgcg ttcatggaga
1681agtacactgt gccccacaag cagggctggg tctggtcaat gcccaagttc atgaggagga
1741acaagacccc agattaccgg ggacagcacg tatttggggt gccacccctc atccacgtgc
1801agcgcacggg ccagccactg ccacagagca ttcagcaagc catgcgctac ttgcgcagcc
1861agtgcctgga ccaagtaggc atcttccgca agtctggggt caagtccagg atccagaacc
1921tgcgtcaaat gaatgagacc tcgcctgaca atgtctgcta cgagggccag tcagcctacg
1981acgtggctga cctgctaaag cagtatttcc gggacctgcc tgagcccatc ttcaccagca
2041agctcaccac cactttcctc cagatctacc agctcctccc caaggatcag tggttggcag
2101cagcacaagc cgccaccttg ctgctccccg atgagaaccg agaggtgcta cagaccctgc
2161tctacttctt aagtgacatt gcctctgccg aggaaaacca gatgacagca ggcaacctgg
2221cagtgtgcct ggcgccctcc atcttccacc tcaatgtctc taagaaggat agcccctctc
2281ccaggatcaa gagcaaacgc agcctcattg gcaggccagg ccctagggac ctgagtgaca
2341acatggcagc cacccagggc ctgtcgcaca tgatcagtga ctgcaagaaa cttttccagg
2401tgccccagga catggtgctg cagctgtgca gctcctacag cgcagctgag ctcagccctc
2461ccggcccagc cctggctgag ctgcgtcagg cccaagctgc aggggtaagc ctgagcctct
2521acatggaaga gaatatccag gacctgctgc gtgatgctgc tgagcgcttc aagggctgga
2581tgagcgtgcc agggccccag cacacggagc tggcttgcag gaaggcaccg gatgggcacc
2641ccctgcggct atggaaggca tccacagagg tggcagcccc cccagctgtg gtgctgcatc
2701gtgttctccg ggagcgggcc ctctgggatg aggatctgct gcgggcccag gtgctggaag
2761ccctgatgcc gggtgtggag ctgtaccact atgtcaccga cagcatggca ccccatccct
2821gccgcgactt tgtggtgctt cggatgtggc gctctgacct gcctcgtggg ggttgcctgc
2881ttgtctccca gtccctggat ccggaacaac ctgtgccaga gtcgggtgtg cgagccctca
2941tgctcacatc ccagtacctc atggagcctt gcggcttggg ccgctctcgg ctcacacaca
3001tctgccgggc tgacctcagg ggccgttctc ctgactggta caacaaagtc tttggacacc
3061tgtgtgccat ggaagtggca aagatccggg actccttccc caccctgcag gcagcgggcc
3121ctgagacaaa gctgtgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
3181ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
3241cgacaatcaa cctctggatt a

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