Construct: ORF ccsbBroad304_02249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007749.1_s304c1, BRDN0000403010
- Derived from:
- ccsbBroadEn_02249
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFCAB14 (9813)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02249
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9813 | EFCAB14 | EF-hand calcium binding dom... | NM_014774.3 | 100% | 100% | |
| 2 | mouse | 230648 | Efcab14 | EF-hand calcium binding dom... | XM_006503006.3 | 84.1% | 79.5% | (many diffs) |
| 3 | mouse | 230648 | Efcab14 | EF-hand calcium binding dom... | NM_172698.2 | 76.9% | 72.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1551
- ORF length:
- 1485
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa aaagcgcaaa gagctcaatg cattgattgg tttggctggg gacagccgga 121 gaaagaagcc caagaaaggc ccaagcagtc accgcctgct tcgcactgag cctcccgact 181 cagactctga gtccagctcc gaagaggaag aggaattcgg tgtggttgga aatcgctctc 241 gctttgccaa gggagactat ttacgatgct gcaagatctg ttatccgctc tgtggttttg 301 tcatccttgc tgcctgtgtt gtggcctgtg ttggcttggt gtggatgcag gttgctctca 361 aggaggatct ggatgccctc aaggaaaaat ttcgaacaat ggaatctaat cagaaaagct 421 cattccaaga aatccccaaa cttaatgaag aactactcag caagcaaaaa caacttgaga 481 agattgaatc tggagagatg ggtttgaaca aagtctggat aaacatcaca gaaatgaata 541 agcagatttc tctgttgact tctgcagtga accacctcaa agccaatgtt aagtcagctg 601 cagacttgat tagcctgcct accactgtag agggacttca gaagagtgta gcttccattg 661 gcaatacttt aaacagcgtc catcttgctg tggaagcact acagaaaact gtggatgaac 721 acaagaaaac gatggaatta ctgcagagtg atatgaatca gcacttcttg aaggagactc 781 ctggaagcaa ccagatcatt ccgtcacctt cagccacatc agaacttgac aataaaaccc 841 acagtgagaa tttgaaacag gatatcctgt accttcacaa ctctttagag gaggtaaaca 901 gtgccctagt ggggtaccag agacagaatg atcttaaact cgagggaatg aacgagacag 961 tcagtaatct tacccagaga gtcaacctga tagaaagcga tgtggttgct atgagcaagg 1021 tagaaaagaa agcaaacctg tccttcagca tgatgggtga tagatctgcc actctgaaaa 1081 gacagtcttt ggatcaagtc accaacagaa cagatacagt aaaaatccaa agcataaaga 1141 aagaagatag ttcaaattct caggtatcca agctaagaga gaaactccag ctgatcagtg 1201 ctcttacaaa caaacctgag agcaacaggc ctccagagac cgccgatgaa gagcaagtag 1261 agagtttcac atcaaagcca tcagcattgc caaaattttc acagtttctt ggagacccag 1321 ttgagaaagc tgcccaacta agacctatct ccctaccagg agtttctagc actgaagatc 1381 ttcaggattt attccgcaag actggccagg acgtggatgg gaagctgacc taccaggaaa 1441 tctggacctc cctaggttct gctatgccag aaccagagag cttgagagca tttgattccg 1501 atggagatgg aagatactca ttcctggagc taagggtagc tttaggtatc tacccaactt 1561 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1621 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta