Construct: ORF ccsbBroad304_02288
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000879.1_s304c1, BRDN0000399464
- Derived from:
- ccsbBroadEn_02288
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNE3 (10008)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02288
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10008 | KCNE3 | potassium voltage-gated cha... | NM_005472.4 | 100% | 100% | |
| 2 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_017017047.1 | 100% | 100% | |
| 3 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_017017048.1 | 100% | 100% | |
| 4 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_017017049.1 | 100% | 100% | |
| 5 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_017017051.2 | 100% | 100% | |
| 6 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_017017052.1 | 100% | 100% | |
| 7 | human | 10008 | KCNE3 | potassium voltage-gated cha... | XM_011544713.2 | 70% | 70% | 1_132del |
| 8 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | NM_001190869.1 | 86.7% | 93.2% | (many diffs) |
| 9 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | NM_001190870.1 | 86.7% | 93.2% | (many diffs) |
| 10 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | NM_001190871.2 | 86.7% | 93.2% | (many diffs) |
| 11 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | NM_001190950.1 | 86.7% | 93.2% | (many diffs) |
| 12 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | NM_020574.5 | 86.7% | 93.2% | (many diffs) |
| 13 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | XM_006508058.2 | 86.7% | 93.2% | (many diffs) |
| 14 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | XM_006508059.2 | 86.7% | 93.2% | (many diffs) |
| 15 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | XM_006508062.1 | 86.7% | 93.2% | (many diffs) |
| 16 | mouse | 57442 | Kcne3 | potassium voltage-gated cha... | XM_017322388.1 | 86.7% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 375
- ORF length:
- 309
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gactaccaat ggaacggaga cctggtatga gagcctgcat gccgtgctga 121 aggctctaaa tgccactctt cacagcaatt tgctctgccg gccagggcca gggctggggc 181 cagacaacca gactgaagag aggcgggcca gcctacctgg ccgtgatgac aactcctaca 241 tgtacattct ctttgtcatg tttctatttg ctgtaactgt gggcagcctc atcctgggat 301 acacccgctc ccgcaaagtg gacaagcgta gtgaccccta tcatgtgtat atcaagaacc 361 gtgtgtctat gatctaccca actttcttgt acaaagtggt tggtaagcct atccctaacc 421 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 481 cgacaatcaa cctctggatt a