Construct: ORF ccsbBroad304_02378

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011056.1_s304c1, BRDN0000409994
Derived from:
ccsbBroadEn_02378
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STUB1 (10273)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02378
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10273 STUB1 STIP1 homology and U-box co... NM_005861.4 100% 100%
2 human 10273 STUB1 STIP1 homology and U-box co... NM_001293197.2 76.2% 76.2% 0_1ins216
3 mouse 56424 Stub1 STIP1 homology and U-Box co... NM_019719.3 88.7% 97.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
975
ORF length:
909
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa gggcaaggag gagaaggagg gcggcgcacg gctgggcgct ggcggcggaa
121gccccgagaa gagcccgagc gcgcaggagc tcaaggagca gggcaatcgt ctgttcgtgg
181gccgaaagta cccggaggcg gcggcctgct acggccgcgc gatcacccgg aacccgctgg
241tggccgtgta ttacaccaac cgggccttgt gctacctgaa gatgcagcag cacgagcagg
301ccctggccga ctgccggcgc gccctggagc tggacgggca gtctgtgaag gcgcacttct
361tcctggggca gtgccagctg gagatggaga gctatgatga ggccatcgcc aatctgcagc
421gagcttacag cctggccaag gagcagcggc tgaacttcgg ggacgacatc cccagcgctc
481ttcgaatcgc gaagaagaag cgctggaaca gcattgagga gcggcgcatc caccaggaga
541gcgagctgca ctcctacctc tccaggctca ttgccgcgga gcgtgagagg gagctggaag
601agtgccagcg aaaccacgag ggtgatgagg acgacagcca cgtccgggcc cagcaggcct
661gcattgaggc caagcacgac aagtacatgg cggacatgga cgagcttttt tctcaggtgg
721atgagaagag gaagaagcga gacatccccg actacctgtg tggcaagatc agctttgagc
781tgatgcggga gccgtgcatc acgcccagtg gcatcaccta cgaccgcaag gacatcgagg
841agcacctgca gcgtgtgggt cattttgacc ccgtgacccg gagccccctg acccaggaac
901agctcatccc caacttggct atgaaggagg ttattgacgc attcatctct gagaatggct
961gggtggagga ctactgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1021ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1081cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)