Construct: ORF ccsbBroad304_02378
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011056.1_s304c1, BRDN0000409994
- Derived from:
- ccsbBroadEn_02378
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STUB1 (10273)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02378
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10273 | STUB1 | STIP1 homology and U-box co... | NM_005861.4 | 100% | 100% | |
| 2 | human | 10273 | STUB1 | STIP1 homology and U-box co... | NM_001293197.2 | 76.2% | 76.2% | 0_1ins216 |
| 3 | mouse | 56424 | Stub1 | STIP1 homology and U-Box co... | NM_019719.3 | 88.7% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 975
- ORF length:
- 909
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gggcaaggag gagaaggagg gcggcgcacg gctgggcgct ggcggcggaa 121 gccccgagaa gagcccgagc gcgcaggagc tcaaggagca gggcaatcgt ctgttcgtgg 181 gccgaaagta cccggaggcg gcggcctgct acggccgcgc gatcacccgg aacccgctgg 241 tggccgtgta ttacaccaac cgggccttgt gctacctgaa gatgcagcag cacgagcagg 301 ccctggccga ctgccggcgc gccctggagc tggacgggca gtctgtgaag gcgcacttct 361 tcctggggca gtgccagctg gagatggaga gctatgatga ggccatcgcc aatctgcagc 421 gagcttacag cctggccaag gagcagcggc tgaacttcgg ggacgacatc cccagcgctc 481 ttcgaatcgc gaagaagaag cgctggaaca gcattgagga gcggcgcatc caccaggaga 541 gcgagctgca ctcctacctc tccaggctca ttgccgcgga gcgtgagagg gagctggaag 601 agtgccagcg aaaccacgag ggtgatgagg acgacagcca cgtccgggcc cagcaggcct 661 gcattgaggc caagcacgac aagtacatgg cggacatgga cgagcttttt tctcaggtgg 721 atgagaagag gaagaagcga gacatccccg actacctgtg tggcaagatc agctttgagc 781 tgatgcggga gccgtgcatc acgcccagtg gcatcaccta cgaccgcaag gacatcgagg 841 agcacctgca gcgtgtgggt cattttgacc ccgtgacccg gagccccctg acccaggaac 901 agctcatccc caacttggct atgaaggagg ttattgacgc attcatctct gagaatggct 961 gggtggagga ctactgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 1021 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1081 cgacaatcaa cctctggatt a