Construct: ORF ccsbBroad304_02386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017679.1_s304c1, BRDN0000402641
- Derived from:
- ccsbBroadEn_02386
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF1B (10289)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02386
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10289 | EIF1B | eukaryotic translation init... | NM_005875.3 | 100% | 100% | |
| 2 | human | 10209 | EIF1 | eukaryotic translation init... | NM_005801.4 | 82% | 92% | (many diffs) |
| 3 | mouse | 68969 | Eif1b | eukaryotic translation init... | NM_026892.3 | 92.3% | 100% | (many diffs) |
| 4 | mouse | 20918 | Eif1 | eukaryotic translation init... | NM_011508.2 | 81.7% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 405
- ORF length:
- 339
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc cactatccag aacctccaat ctttcgaccc ctttgctgat gcaactaagg 121 gtgacgactt actcccggca gggactgagg attacattca tataagaatc cagcaacgga 181 acggcagaaa gacactgact actgttcagg gcattgcaga tgattatgac aaaaagaaac 241 ttgtgaaagc tttcaaaaag aaatttgcct gtaatggtac tgtgattgaa catcctgaat 301 acggagaggt tattcagctt caaggtgacc aaagaaaaaa catctgccag tttctcttgg 361 aggttggcat tgtaaaggag gaacagctta aggttcatgg attctaccca actttcttgt 421 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 481 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a