Construct: ORF ccsbBroad304_02404
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015602.1_s304c1, BRDN0000411775
- Derived from:
- ccsbBroadEn_02404
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRVI1 (10335)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02404
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10335 | MRVI1 | murine retrovirus integrati... | NM_001100167.2 | 100% | 100% | |
| 2 | human | 10335 | MRVI1 | murine retrovirus integrati... | NM_001206881.1 | 100% | 100% | |
| 3 | human | 10335 | MRVI1 | murine retrovirus integrati... | NM_001206880.1 | 77.2% | 64.4% | (many diffs) |
| 4 | human | 10335 | MRVI1 | murine retrovirus integrati... | NM_001100163.2 | 72.7% | 72.7% | 1_672del |
| 5 | human | 10335 | MRVI1 | murine retrovirus integrati... | NM_001098579.2 | 66% | 66% | 1_921del |
| 6 | human | 10335 | MRVI1 | murine retrovirus integrati... | NM_130385.3 | 65.4% | 65.4% | 1_945del |
| 7 | mouse | 17540 | Mrvi1 | MRV integration site 1 | XM_006507418.3 | 82.5% | 83.7% | (many diffs) |
| 8 | mouse | 17540 | Mrvi1 | MRV integration site 1 | NM_194464.3 | 58.8% | 59.5% | (many diffs) |
| 9 | mouse | 17540 | Mrvi1 | MRV integration site 1 | XM_006507415.2 | 58.8% | 59.5% | (many diffs) |
| 10 | mouse | 17540 | Mrvi1 | MRV integration site 1 | XM_006507416.3 | 58.8% | 59.5% | (many diffs) |
| 11 | mouse | 17540 | Mrvi1 | MRV integration site 1 | XM_011241686.1 | 57.6% | 58.4% | (many diffs) |
| 12 | mouse | 17540 | Mrvi1 | MRV integration site 1 | NM_010826.5 | 54.7% | 55.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1860
- ORF length:
- 1791
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccctgaac agccctcagc ctggccccgt ggagagcgag ctggggaagc 121 agctcttgaa aacgggctgg gagggcagcc ctctgccgag aagtccaacc caggatgcgg 181 caggagtggg tcccccagcc tcccagggga gaggcccagc tggagagccg atggggcccg 241 aggctggctc caaagctgag cttccaccca ctgtgtcccg gcccccgctg ctgcgagggc 301 tctcctggga cagtggccct gaagaacctg gcccccggct gcagaaagtg cttgccaagc 361 tgccactggc agaggaagaa aagcgttttg caggcaaggc cggcggcaag ctggccaagg 421 cccctggtct caaagacttt cagatacaag tgcagcccgt gcggatgcag aaactgacca 481 agctccgaga ggagcacatc ctgatgagaa atcagaactt agtggggctc aagcttccag 541 accttagtga agcagctgag caggaaaaag ggcttccttc tgaactctcc ccagctattg 601 aggaagaaga gtcaaagagt ggcttagatg tcatgcctaa tatttctgat gtgctgctgc 661 gcaaactgcg ggtccacagg agtctccctg gaagtgcccc tccactcact gaaaaggaag 721 ttgagaacgt gtttgtgcaa ctgtccttgg cctttagaaa tgacagctac actctggaat 781 ctagaattaa ccaggctgaa agggaacgca acctgacaga ggagaacact gagaaagaac 841 tggaaaactt caaagcttcc attacgtcct cagcttcact ctggcaccac tgtgagcacc 901 gggaaaccta ccagaagttg ctggaggaca tcgctgtcct gcaccgcctg gctgcccgcc 961 tctccagccg agctgaggtg gtaggcgccg tccgccagga aaagcgcatg tcgaaagcaa 1021 cggaagtgat gatgcagtat gtggagaatc taaagaggac gtatgagaag gaccatgcgg 1081 agctcatgga gtttaaaaag cttgcaaatc agaattcaag ccgcagctgt ggcccctctg 1141 aagatggggt ccctcgcacg gcacggtcca tgtccctcac gctgggaaag aatatgcctc 1201 gccggagggt cagcgttgct gtggttccta agtttaatgc cctgaatctg cctggccaaa 1261 ctcccagctc atcatccatt ccctccttac cagccttgtc ggaatcaccc aatgggaaag 1321 gcagcctacc tgtcacttca gcactgcctg cacttttgga aaatggaaag acaaatgggg 1381 acccagattg tgaagcctct gctcctgcgc tgaccctgag ctgcctggag gagcttagtc 1441 aggagaccaa ggccaggatg gaggaagaag cctacagcaa gggattccaa gaaggtctaa 1501 agaagaccaa agaacttcaa gacctgaagg aggaggagga agaacagaag agtgagagtc 1561 ctgaggaacc tgaagaggta gaagaaactg aggaagagga aaagggccca agaagcagca 1621 aacttgaaga attggtccat ttcttacaag tcatgtatcc caaactgtgt cagcactggc 1681 aagtgatctg gatgatggct gcagtgatgc tggtcttgac tgttgtgctg gggctctaca 1741 attcctataa ctcttgtgca gagcaggctg atgggcccct tggaagatcc acttgctcgg 1801 cagcccagag ggactcctgg tggagctcag gactccagca tgagcagcct acagagcagt 1861 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1921 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1981 ggatta