Construct: ORF ccsbBroad304_02409
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003926.1_s304c1, BRDN0000398916
- Derived from:
- ccsbBroadEn_02409
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMG20A (10363)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02409
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10363 | HMG20A | high mobility group 20A | NM_001304504.2 | 100% | 100% | |
| 2 | human | 10363 | HMG20A | high mobility group 20A | NM_018200.3 | 100% | 100% | |
| 3 | human | 10363 | HMG20A | high mobility group 20A | XM_011521158.3 | 100% | 100% | |
| 4 | human | 10363 | HMG20A | high mobility group 20A | NM_001304505.1 | 67.4% | 67.4% | 0_1ins339 |
| 5 | human | 10363 | HMG20A | high mobility group 20A | XR_001751049.2 | 47% | 1_304del;1346_2211del | |
| 6 | mouse | 66867 | Hmg20a | high mobility group 20A | NM_025812.3 | 91.3% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1107
- ORF length:
- 1041
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga aaacttgatg actagctcca ccctaccgcc cctttttgca gatgaagacg 121 gttccaagga gagtaatgat ctggctacca ctgggttaaa tcacccagag gttccataca 181 gtagtggcgc cacatcatcc accaacaatc cagaatttgt ggaggatctc tctcaaggtc 241 agttgcttca gagtgagtct tcaaatgcag cagaaggcaa tgaacagagg catgaagatg 301 agcaacgaag taaacgagga ggttggtcca aaggaagaaa gaggaagaaa cctcttcgag 361 acagcaatgc acccaaatcc ccccttacag gatatgttcg gttcatgaat gagcgtcgag 421 aacaacttcg agcaaagaga ccagaagtcc catttccaga aatcacaagg atgttaggca 481 atgaatggag taaactgcct cctgaggaaa aacagcgcta ccttgatgaa gcagacagag 541 ataaggagcg ttacatgaag gaactggaac agtatcagaa aacagaggcc tacaaggtct 601 tcagtaggaa aacccaggac cgtcagaaag gcaaatctca taggcaagat gcagcccggc 661 aggccactca tgatcatgag aaagaaacag aggtaaagga acggtctgtt tttgacatcc 721 ctatatttac agaggaattc ttgaaccata gcaaagctcg ggaagcagag ctccgccagc 781 ttcgcaaatc caacatggag tttgaggaga ggaatgcagc cctgcaaaag cacgtggaga 841 gcatgcgcac agcagtggag aagctggagg tggatgtgat ccaggagcgg agccgcaaca 901 cagtcttaca gcagcacctg gagaccctgc ggcaggtgct gaccagcagc tttgccagca 961 tgcccttgcc tggaagtgga gagacaccta cagtggacac cattgactca tatatgaaca 1021 gactgcacag tattatttta gctaatcccc aagacaatga aaacttcata gctacagttc 1081 gagaagttgt gaacagactc gatcgttacc caactttctt gtacaaagtg gttggtaagc 1141 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1201 ggcgcgttaa gtcgacaatc aacctctgga tta