Construct: ORF ccsbBroad304_02435

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008102.1_s304c1, BRDN0000406862
Derived from:
ccsbBroadEn_02435
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
N4BP2L2 (10443)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02435
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 NM_014887.3 100% 100%
2 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_017020347.2 100% 100%
3 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_017020348.2 100% 100%
4 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_017020349.1 100% 100%
5 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_011534874.3 97.5% 97% 1699_1699delAinsTGGGGAGGCTCTCT;1703T>C;1710_1710delTins27
6 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 NM_001320836.2 48.7% 47.8% (many diffs)
7 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_017020328.2 48.7% 47.8% (many diffs)
8 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_017020329.2 48.7% 47.8% (many diffs)
9 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_017020330.2 48.7% 47.8% (many diffs)
10 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_017020324.2 48.2% 47.3% (many diffs)
11 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_017020325.2 48.2% 47.3% (many diffs)
12 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_017020326.1 48.2% 47.3% (many diffs)
13 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_017020327.1 48.2% 47.3% (many diffs)
14 human 10443 N4BP2L2 NEDD4 binding protein 2 like 2 XM_024449305.1 48.2% 47.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1815
ORF length:
1749
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggaatgtc ttatggtgaa attgaaggta aattcttggg acctagagaa gaagtaacga
121gtgagccacg ctgtaaaaaa ttgaagtcaa ccacagagtc gtatgttttt cacaatcata
181gtaatgctga ttttcacaga atccaagaga aaactggaaa tgattgggtc cctgtgacca
241tcattgatgt cagaggacat agttatttgc aggagaacaa aatcaaaact acagatttgc
301atagaccttt gcatgatgag atgcctggta atagaccaga tgttattgaa tccattgatt
361cacaggtttt acaggaagca cgtcctccat tagtatccgc agacgatgag atatatagca
421caagtaaagc atttatagga cccatttaca aaccccctga gaaaaagaaa cgtaatgaag
481ggaggaatga ggcacatgtt ctaaatggta taaatgacag aggaggacaa aaagagaaac
541agaaatttaa ctctgaaaaa tcagagattg acaatgaatt attccagttt tacaaagaaa
601ttgaagagct tgaaaaggaa aaagatggtt ttgagaacag ttgtaaagaa tctgaacctt
661ctcaggaaca atttgttcca ttttatgagg gtcataataa tggtctctta aaacctgatg
721aagaaaagaa agatcttagt aataaagcta tgccatcaca ttgtgattat cagcagaact
781tggggaatga gccagacaaa tatccctgta atggacaagt aatacctaca ttttgtgaca
841cttcatttac ttctttcagg cctgaatggc agtcagtata tccttttata gtgccctatg
901gtccccctct tcccagtttg aactatcatt taaacattca gagattcagt ggtccaccaa
961atccaccatc aaatattttc caagcccaag atgactctca gatacaaaat ggatattatg
1021taaataattg tcatgttaac tggaattgca tgacttttga tcagaacaat gaatatactg
1081actgtagtga gaataggagt agtgttcatc cctctggaaa tggctgcagt atgcaagatc
1141gatatgtgag taatggtttc tgtgaagtca gagaaagatg ctggaaagat cattgtatgg
1201acaagcataa tggaacagac aggtttgtga accagcagtt tcaagaggaa aagttaaata
1261aattgcagaa gttacttatt cttttaagag gtctgcctgg ttctgggaaa acaacattgt
1321ctcgaattct gcttggtcag aatcgtgatg gcattgtgtt cagcactgat gactattttc
1381accatcaaga tgggtacagg tataatgtta atcaacttgg tgatgcccat gactggaacc
1441agaacagagc aaaacaagct atcgatcagg gaagatctcc agttataata gataacacta
1501atatacaagc ttgggaaatg aagccatatg tggaagtggc cataggaaaa ggatacagag
1561tagagtttca tgaacctgaa acttggtgga aatttgatcc tgaagaatta gaaaagagga
1621ataaacatgg tgtgtctcga aagaagattg ctcagatgtt ggatcgttat gaatatcaaa
1681tgtccatttc tattgtaatg aattcagtgg aaccatcaca caaaagcaca caaagacctc
1741ctcctccaca ggggagacag aggtggggag gctctcttgg ctcacataat cgtgtctgtg
1801tcacaaataa tcattaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1861ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1921cgacaatcaa cctctggatt a

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