Construct: ORF ccsbBroad304_02450
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015443.1_s304c1, BRDN0000411555
- Derived from:
- ccsbBroadEn_02450
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF3M (10480)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02450
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10480 | EIF3M | eukaryotic translation init... | NM_006360.6 | 100% | 100% | |
| 2 | human | 10480 | EIF3M | eukaryotic translation init... | XM_011519840.3 | 83.1% | 83.1% | 0_1ins189 |
| 3 | human | 10480 | EIF3M | eukaryotic translation init... | NM_001307929.2 | 63.5% | 61.2% | (many diffs) |
| 4 | mouse | 98221 | Eif3m | eukaryotic translation init... | NM_145380.2 | 91.7% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1188
- ORF length:
- 1122
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cgtcccggcc ttcatcgaca tcagtgaaga agatcaggct gctgagcttc 121 gtgcttatct gaaatctaaa ggagctgaga tttcagaaga gaactcggaa ggtggacttc 181 atgttgattt agctcaaatt attgaagcct gtgatgtgtg tctgaaggag gatgataaag 241 atgttgaaag tgtgatgaac agtgtggtat ccctactctt gatcctggaa ccagacaagc 301 aagaagcttt gattgaaagc ctatgtgaaa agctggtcaa atttcgcgaa ggtgaacgcc 361 cgtctctgag actgcagttg ttaagcaacc ttttccacgg gatggataag aatactcctg 421 taagatacac agtgtattgc agccttatta aagtggcagc atcttgtggg gccatccagt 481 acatcccaac tgagctggat caagttagaa aatggatttc tgactggaat ctcaccactg 541 aaaaaaagca caccctttta agactacttt atgaggcact tgtggattgt aagaagagtg 601 atgctgcttc aaaagtcatg gtggaattgc tcggaagtta cacagaggac aatgcttccc 661 aggctcgagt tgatgcccac aggtgtattg tacgagcatt gaaagatcca aatgcatttc 721 tttttgacca ccttcttact ttaaaaccag tcaagttttt ggaaggcgag cttattcatg 781 atcttttaac catttttgtg agtgctaaat tggcatcata tgtcaagttt tatcagaata 841 ataaagactt cattgattca cttggcctgt tacatgaaca gaatatggca aaaatgagac 901 tacttacttt tatgggaatg gcagtagaaa ataaggaaat ttcttttgac acaatgcagc 961 aagaacttca gattggagct gatgatgttg aagcatttgt tattgacgcc gtaagaacta 1021 aaatggtcta ctgcaaaatt gatcagaccc agagaaaagt agttgtcagt catagcacac 1081 atcggacatt tggaaaacag cagtggcaac aactgtatga cacacttaat gcctggaaac 1141 aaaatctgaa caaagtgaaa aacagccttt tgagtctttc tgatacctgc ccaactttct 1201 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1261 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta