Construct: ORF ccsbBroad304_02502
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002618.1_s304c1, BRDN0000399870
- Derived from:
- ccsbBroadEn_02502
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNA13 (10672)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02502
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10672 | GNA13 | G protein subunit alpha 13 | NM_006572.6 | 100% | 100% | |
| 2 | human | 10672 | GNA13 | G protein subunit alpha 13 | NM_001282425.1 | 74.8% | 74.8% | 0_1ins285 |
| 3 | human | 10672 | GNA13 | G protein subunit alpha 13 | XM_011524202.1 | 50.7% | 50.1% | (many diffs) |
| 4 | mouse | 14674 | Gna13 | guanine nucleotide binding ... | NM_010303.3 | 90.7% | 96.8% | (many diffs) |
| 5 | mouse | 14674 | Gna13 | guanine nucleotide binding ... | XM_006532229.2 | 41.4% | 43.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacttcctg ccgtcgcggt ccgtgctgtc cgtgtgcttc cccggctgcc 121 tgctgacgag tggcgaggcc gagcagcaac gcaagtccaa ggagatcgac aaatgcctgt 181 ctcgggaaaa gacctatgtg aagcggctgg tgaagatcct gctgctgggc gcgggcgaga 241 gcggcaagtc caccttcctg aagcagatgc ggatcatcca cgggcaggac ttcgaccagc 301 gcgcgcgcga ggagttccgc cccaccatct acagcaacgt gatcaaaggt atgagggtgc 361 tggttgatgc tcgagagaag cttcatattc cctggggaga caactcaaac caacaacatg 421 gagataagat gatgtcgttt gatacccggg cccccatggc agcccaagga atggtggaaa 481 caagggtttt cttacaatat cttcctgcta taagagcaTT ATGGGCAGAC AGCGGCATAC 541 AGAATGCCTA TGACCGGCGT CGAGAATTTC AACTGGGTGA ATCTGTAAAA TATTTCCTGG 601 ATAACTTGGA TAAACTTGGA GAACCAGATT ATATTCCATC ACAACAAGAT ATTCTGCTTG 661 CCAGAAGACC CACCAAAGGC ATCCATGAAT ACGACTTTGA AATAAAAAAT GTTCCTTTCA 721 AAATGGTTGA TGTAGGTGGT CAGAGATCAG AAAGGAAACG TTGGTTTGAA TGTTTCGACA 781 GTGTGACATC AATACTTTTC CTTGTTTCCT CAAGTGAATT TGACCAGGTG CTTATGGAAG 841 ATCGACTGAC CAATCGCCTT ACAGAGTCTC TGAACATTTT TGAAACAATC GTCAATAACC 901 GGGTTTTCAG CAATGTCTCC ATAATTCTGT TCTTAAACAA GACAGACTTG CTTGAGGAGA 961 AGGTGCAAAT TGTGAGCATC AAAGACTATT TCCTAGAATT TGAAGGGGAT CCCCACTGCT 1021 TAAGAGACGT CCAAAAATTC CTGGTGGAAT GTTTCCGGAA CAAACGCCGG GACCAGCAAC 1081 AGAAGCCCTT ATACCACCAC TTCACCACTG CTATCAACAC GGAGAACATC CGCCTTGTTT 1141 TCCGTGACGT GAAGGATACT ATTCTGCATG ACAACCTCAA GCAGCTTATG CTACAGTGCC 1201 CAACTTTCTT GTACaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1261 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1321 tta