Construct: ORF ccsbBroad304_02532
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012825.1_s304c1, BRDN0000404300
- Derived from:
- ccsbBroadEn_02532
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STARD10 (10809)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02532
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10809 | STARD10 | StAR related lipid transfer... | NM_006645.2 | 100% | 100% | |
| 2 | mouse | 56018 | Stard10 | START domain containing 10 | NM_019990.4 | 91.1% | 97.2% | (many diffs) |
| 3 | mouse | 56018 | Stard10 | START domain containing 10 | XM_011241865.1 | 72.8% | 77.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 939
- ORF length:
- 873
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gaagctggcg gcctctacag agccccaagg gcctcggccg gtcctgggcc 121 gtgagagtgt ccaggtgccc gatgaccaag actttcgcag cttccggtca gagtgtgagg 181 ctgaggtggg ctggaacctg acctatagca gggctggggt gtctgtctgg gtgcaggctg 241 tggagatgga tcggacgctg cacaagatca agtgccggat ggagtgctgt gatgtgccag 301 ccgagacact ctacgacgtc ctacacgaca ttgagtaccg caagaaatgg gacagcaacg 361 tcattgagac ttttgacatc gcccgcttga cagtcaacgc tgacgtgggc tattactcct 421 ggaggtgtcc caagcccctg aagaaccgtg atgtcatcac cctccgctcc tggctcccca 481 tgggcgctga ttacatcatt atgaactact cagtcaaaca tcccaaatac ccacctcgga 541 aagacttggt ccgagctgtg tccatccaga cgggctacct catccagagc acagggccca 601 agagctgcgt catcacctac ctggcccagg tggaccccaa aggctcctta cccaagtggg 661 tggtgaataa atcttctcag ttcctggctc ccaaggccat gaagaagatg tacaaggcgt 721 gcctcaagta ccccgagtgg aaacagaagc acctgcctca cttcaagccg tggctgcacc 781 cggagcagag cccgttgccg agcctggcgc tgtcggagct gtcggtgcag catgcggact 841 cactggagaa catcgacgag agcgcggtgg ccgagagcag agaggagcgg atgggcggcg 901 cgggcggcga gggcagcgac gacgacacct cgctcacctg cccaactttc ttgtacaaag 961 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1021 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta