Construct: ORF ccsbBroad304_02575
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002874.1_s304c1, BRDN0000460647
- Derived from:
- ccsbBroadEn_02575
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERP29 (10961)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02575
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10961 | ERP29 | endoplasmic reticulum prote... | NM_006817.4 | 100% | 100% | |
| 2 | human | 10961 | ERP29 | endoplasmic reticulum prote... | XM_017018720.1 | 61.3% | 61.3% | 0_1ins303 |
| 3 | human | 10961 | ERP29 | endoplasmic reticulum prote... | NM_001034025.1 | 19.9% | 18.7% | (many diffs) |
| 4 | mouse | 67397 | Erp29 | endoplasmic reticulum prote... | NM_026129.2 | 86.7% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 852
- ORF length:
- 783
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgccgct gtgccccgcg ccgcatttct ctccccgctg cttccccttc 121 tcctgggctt cctgctcctc tccgctccgc atggcggcag cggcctgcac accaagggcg 181 cccttcccct ggatacggtc actttctaca aggtcattcc caaaagcaag ttcgtcttgg 241 tgaagttcga cacccagtac ccctacggtg agaagcagga tgagttcaag cgtcttgctg 301 aaaactcggc ttccagcgat gatctcttgg tggcagaggt ggggatctca gattatggtg 361 acaagctgaa catggagctg agtgagaaat acaagctgga caaagagagc tacccagtct 421 tctacctctt ccgggatggg gactttgaga acccagtccc atacactggg gcagttaagg 481 ttggagccat ccagcgctgg ctgaaggggc aaggggtcta cctaggtatg cctggttgcc 541 tgcctgtata cgacgccctg gccggggagt tcatcagggc ctctggtgtg gaggcccgcc 601 aggccctctt gaagcagggg caagataacc tctcaagtgt gaaggagact cagaagaagt 661 gggccgagca atacctgaag atcatgggga agatcttaga ccaaggggag gacttcccag 721 catcagagat gacacggatc gccaggctga ttgagaagaa caagatgagt gacgggaaga 781 aggaggagct ccagaagagc ttaaacatcc tgactgcctt ccagaagaag ggggccgaga 841 aagaggagct gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 901 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 961 caatcaacct ctggatta