Construct: ORF ccsbBroad304_02577
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017240.1_s304c1, BRDN0000410314
- Derived from:
- ccsbBroadEn_02577
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STIP1 (10963)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02577
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10963 | STIP1 | stress induced phosphoprote... | NM_006819.3 | 100% | 100% | |
| 2 | human | 10963 | STIP1 | stress induced phosphoprote... | NM_001282653.2 | 95.5% | 95.5% | 217_218ins72 |
| 3 | human | 10963 | STIP1 | stress induced phosphoprote... | NM_001282652.1 | 91.9% | 91.5% | 1_135delinsA;142_149delCTGAGATGinsA |
| 4 | mouse | 20867 | Stip1 | stress-induced phosphoprote... | NM_016737.2 | 88.2% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1695
- ORF length:
- 1629
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gcaggtcaat gagctgaagg agaaaggcaa caaggccctg agcgtgggta 121 acatcgatga tgccttacag tgctactccg aagctattaa gctggatccc cacaaccacg 181 tgctgtacag caaccgttct gctgcctatg ccaagaaagg agactaccag aaggcttatg 241 aggatggctg caagactgtc gacctaaagc ctgactgggg caagggctat tcacgaaaag 301 cagcagctct agagttctta aaccgctttg aagaagccaa gcgaacctat gaggagggct 361 taaaacacga ggcaaataac cctcaactga aagagggttt acagaatatg gaggccaggt 421 tggcagagag aaaattcatg aaccctttca acatgcctaa tctgtatcag aagttggaga 481 gtgatcccag gacaaggaca ctactcagtg atcctaccta ccgggagctg atagagcagc 541 tacgaaacaa gccttctgac ctgggcacga aactacaaga tccccggatc atgaccactc 601 tcagcgtcct ccttggggtc gatctgggca gtatggatga ggaggaagag attgcaacac 661 ctccaccacc accccctccc aaaaaggaga ccaagccaga gccaatggaa gaagatcttc 721 cagagaataa gaagcaggca ctgaaagaaa aagagctggg gaacgatgcc tacaagaaga 781 aagactttga cacagccttg aagcattacg acaaagccaa ggagctggac cccactaaca 841 tgacttacat taccaatcaa gcagcggtat actttgaaaa gggcgactac aataagtgcc 901 gggagctttg tgagaaggcc attgaagtgg ggagagaaaa ccgagaagac tatcgacaga 961 ttgccaaagc atatgctcga attggcaact cctacttcaa agaagaaaag tacaaggatg 1021 ccatccattt ctataacaag tctctggcag agcaccgaac cccagatgtg ctcaagaaat 1081 gccagcaggc agagaaaatc ctgaaggagc aagagcggct ggcctacata aaccccgacc 1141 tggctttgga ggagaagaac aaaggcaacg agtgttttca gaaaggggac tatccccagg 1201 ccatgaagca ttatacagaa gccatcaaaa ggaacccgaa agatgccaaa ttatacagca 1261 atcgagctgc ctgctacacc aaactcctgg agttccagct ggcactcaag gactgtgagg 1321 aatgtatcca gctggagccg accttcatca agggttatac acggaaagcc gctgcgctgg 1381 aagcgatgaa ggactacacc aaagccatgg atgtgtacca gaaggcgcta gacctggact 1441 ccagctgtaa ggaggcggca gacggctacc agcgctgtat gatggcgcag tacaaccggc 1501 acgacagccc cgaagatgtg aagcgacgag ccatggccga ccctgaggtg cagcagatca 1561 tgagtgaccc agccatgcgc cttatcctgg aacagatgca gaaggacccc caggcactca 1621 gcgaacactt aaagaatcct gtaatagcac agaagatcca gaagctgatg gatgtgggtc 1681 tgattgcaat tcggtgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 1741 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1801 cgacaatcaa cctctggatt a