Construct: ORF ccsbBroad304_02708
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002455.1_s304c1, BRDN0000407146
- Derived from:
- ccsbBroadEn_02708
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM32 (22954)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02708
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22954 | TRIM32 | tripartite motif containing 32 | NM_001099679.2 | 100% | 100% | |
| 2 | human | 22954 | TRIM32 | tripartite motif containing 32 | NM_012210.3 | 100% | 100% | |
| 3 | human | 22954 | TRIM32 | tripartite motif containing 32 | XM_005251813.4 | 100% | 100% | |
| 4 | human | 22954 | TRIM32 | tripartite motif containing 32 | XM_011518398.2 | 100% | 100% | |
| 5 | human | 22954 | TRIM32 | tripartite motif containing 32 | XM_017014486.1 | 100% | 100% | |
| 6 | mouse | 69807 | Trim32 | tripartite motif-containing 32 | NM_001161782.1 | 87.7% | 95.8% | (many diffs) |
| 7 | mouse | 69807 | Trim32 | tripartite motif-containing 32 | NM_053084.2 | 87.7% | 95.8% | (many diffs) |
| 8 | mouse | 69807 | Trim32 | tripartite motif-containing 32 | XM_006538235.1 | 87.7% | 95.8% | (many diffs) |
| 9 | mouse | 69807 | Trim32 | tripartite motif-containing 32 | XM_006538236.3 | 87.7% | 95.8% | (many diffs) |
| 10 | mouse | 69807 | Trim32 | tripartite motif-containing 32 | XM_006538237.1 | 87.7% | 95.8% | (many diffs) |
| 11 | mouse | 69807 | Trim32 | tripartite motif-containing 32 | XM_006538238.1 | 87.7% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2025
- ORF length:
- 1959
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcagcagca gcttctcacc tgaacctgga tgccctccgg gaagtgctag 121 aatgccccat ctgcatggag tccttcacag aagagcagct gcgtcccaag cttctgcact 181 gtggccatac catctgccgc cagtgcctgg agaagctatt ggccagtagc atcaatggtg 241 tccgctgtcc cttttgcagc aagattaccc gcataaccag cttgacccag ctgacagaca 301 atctgacagt gctaaagatc attgatacag ctgggctcag cgaggctgtg gggctgctca 361 tgtgtcggtc ctgtgggcgg cgtctgcccc ggcaattctg ccggagctgt ggtttggtgt 421 tatgtgagcc ctgccgggag gcagaccatc agcctcctgg ccactgtaca ctccctgtca 481 aagaagcagc tgaggagcgg cgtcgggact ttggagagaa gttaactcgt ctgcgggaac 541 ttatggggga gctgcagcgg cggaaggcag ccttggaagg tgtctccaag gaccttcagg 601 caaggtataa agcagttctc caggagtatg ggcatgagga gcgcagggtc caggatgagc 661 tggctcgctc tcggaagttc ttcacaggct ctttggctga agttgagaag tccaatagtc 721 aagtggtaga ggagcagagt tacctgctta acattgcaga ggtgcaggct gtgtctcgct 781 gtgactactt cctggccaag atcaagcagg cagatgtagc actactggag gagacagctg 841 atgaggagga gccagagctc actgccagct tgcctcggga gctcaccctg caagatgtgg 901 agctccttaa ggtaggtcat gttggccccc tccaaattgg acaagctgtt aagaagcccc 961 ggacagttaa cgtggaagat tcctgggcca tggaggccac agcgtctgct gcctctacct 1021 ctgttacttt tagagagatg gacatgagcc cggaggaagt ggttgccagc cctagggcct 1081 cacctgctaa acagcggggt cctgaggcag cctccaatat ccagcagtgc ctctttctca 1141 agaagatggg ggccaaaggc agcactccag gaatgttcaa tcttccagtc agtctctacg 1201 tgaccagtca aggtgaagta ctagtcgctg accgtggtaa ctatcgtata caagtcttta 1261 cccgcaaagg ctttttgaag gaaatccgcc gcagccccag tggcattgat agctttgtgc 1321 taagcttcct tggggcagat ctacccaacc tcactcctct ctcagtggca atgaactgcc 1381 aggggctgat tggtgtgact gacagctatg ataactccct caaggtatat accttggatg 1441 gccactgcgt ggcctgtcac aggagccagc tgagcaaacc atggggtatc acagccttgc 1501 catctggcca gtttgtagta accgatgtgg aaggtggaaa gctttggtgt ttcacagttg 1561 atcgaggatc aggggtggtc aaatacagct gcctatgtag tgctgtgcgg cccaaatttg 1621 tcacctgtga tgctgagggc accgtctact tcacccaggg cttaggcctc aatctggaga 1681 atcggcagaa tgagcaccac ctggagggtg gcttttccat tggctctgta ggccctgatg 1741 ggcagctggg tcgccagatt agccacttct tctcggagaa tgaggatttc cgctgcattg 1801 ctggcatgtg tgtggatgct cgtggtgatc tcatcgtggc tgacagtagt cgcaaggaaa 1861 ttctccattt tcctaagggt gggggctata gtgtccttat tcgagaggga cttacctgtc 1921 cggtgggcat agccctaact cctaaggggc agctgctggt cttggactgt tgggatcatt 1981 gcatcaagat ctacagctac catctgagaa gatattccac cccataccca actttcttgt 2041 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2101 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a