Construct: ORF ccsbBroad304_02727
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016306.1_s304c1, BRDN0000461713
- Derived from:
- ccsbBroadEn_02727
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTLL12 (23170)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02727
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23170 | TTLL12 | tubulin tyrosine ligase lik... | NM_015140.4 | 100% | 100% | |
| 2 | mouse | 223723 | Ttll12 | tubulin tyrosine ligase-lik... | NM_183017.2 | 85.2% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1998
- ORF length:
- 1932
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggccgagcgg ggtcccgagc gccggcctgc ggagcgtagc agcccgggcc 121 agacgccgga ggagggcgcg caggccttgg ccgagttcgc ggcgctgcac ggcccggcgc 181 tgcgcgcttc gggggtcccc gaacgttact ggggccgcct cctgcacaag ctggagcacg 241 aggttttcga cgctggggaa gtgtttggga tcatgcaagt ggaggaggta gaagaggagg 301 aggacgaggc agcccgggag gtgcggaagc agcagcccaa cccggggaac gagctgtgct 361 acaaggtcat cgtgaccagg gagagcgggc tccaggcagc ccaccccaac agcatcttcc 421 tcatcgacca cgcctggacg tgccgtgtgg agcacgcgcg ccagcagctg cagcaggtgc 481 ccgggctgct gcaccgcatg gccaacctga tgggcattga gttccacggt gagctgccca 541 gtacagaggc tgtggccctg gtgctggagg agatgtggaa gttcaaccag acctaccagc 601 tggcccatgg gacagctgag gagaagatgc cggtgtggta tatcatggac gagttcggtt 661 cgcggatcca gcacgcggac gtgcccagct tcgccacggc acccttcttc tacatgccgc 721 agcaggtggc ctacacgctg ctgtggcccc tgagggacct ggacactggc gaggaggtga 781 cccgagactt tgcctacgga gagacggacc ccctgatccg gaagtgcatg ctgctgccct 841 gggcccccac cgacatgctg gacctcagct cttgcacacc cgagccgccc gccgagcact 901 accaggccat tctggaggaa aacaaggaga agctgccact tgacatcaac cccgtggtgc 961 acccccacgg ccacatcttc aaggtctaca cggacgtgca gcaggtggcc agcagcctca 1021 cccacccgcg cttcaccctc acccagagtg aggcggacgc cgacatcctc ttcaacttct 1081 cacacttcaa ggactacagg aaactcagcc aggagaggcc aggcgtgctg ctgaaccagt 1141 tcccctgcga gaacctgctg actgtcaagg actgcctggc ctccatcgcg cgccgggcag 1201 gtggccccga gggcccaccc tggctgcccc gaaccttcaa cctgcgcact gagctgcccc 1261 agtttgtcag ctacttccag cagcgggaaa ggtggggcga ggacaaccac tggatctgca 1321 agccctggaa cctggcgcgc agcctggaca cccacgtcac caagagcctg cacagcatca 1381 tccggcaccg agagagcacc cccaaggttg tgtccaagta catcgaaagt cccgtgttgt 1441 tccttcgaga agacgtggga aaggtcaagt tcgacatccg ctacatcgtg ctgctgcggt 1501 cagtgaggcc cctacggttg ttcgtgtatg atgtgttctg gctgcggttc tccaaccggg 1561 cctttgcact caacgacctg gatgactacg agaagcactt cacggtcatg aactatgacc 1621 cggatgtggt gctgaagcag gtgcactgtg aagagttcat ccccgagttt gagaagcaat 1681 acccagaatt tccctggacg gacgtccagg ctgagatctt ccgggccttc acggagctgt 1741 tccaggtggc ctgtgccaag ccaccacccc tgggcctctg cgactacccc tcatcccggg 1801 ccatgtatgc cgtcgacctc atgctgaagt gggacaacgg cccagatgga aggcgggtga 1861 tgcagccgca gatcctggag gtgaacttca accccgactg tgagcgagcc tgcaggtacc 1921 accccacctt cttcaacgac gtcttcagca ccttgtttct ggaccagccc ggtggctgcc 1981 acgttacctg ccttgtctac ccaactttct tgtacaaagt ggttggtaag cctatcccta 2041 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2101 agtcgacaat caacctctgg atta