Construct: ORF ccsbBroad304_02769
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003930.1_s304c1, BRDN0000412189
- Derived from:
- ccsbBroadEn_02769
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP1B4 (23439)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02769
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23439 | ATP1B4 | ATPase Na+/K+ transporting ... | NM_001142447.3 | 100% | 100% | |
| 2 | human | 23439 | ATP1B4 | ATPase Na+/K+ transporting ... | NM_012069.5 | 98.8% | 98.8% | 316_317insGTCAGAGTTGGA |
| 3 | human | 23439 | ATP1B4 | ATPase Na+/K+ transporting ... | XM_017029381.1 | 73.6% | 71.4% | (many diffs) |
| 4 | mouse | 67821 | Atp1b4 | ATPase, (Na+)/K+ transporti... | NM_133690.3 | 89.4% | 89% | (many diffs) |
| 5 | mouse | 67821 | Atp1b4 | ATPase, (Na+)/K+ transporti... | NM_001290389.1 | 88.4% | 88.2% | (many diffs) |
| 6 | mouse | 67821 | Atp1b4 | ATPase, (Na+)/K+ transporti... | XM_006541533.1 | 57.7% | 58.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1140
- ORF length:
- 1071
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagaaggcaa ctccggtcca gaagggctcc atcctttcct tacagttatc 121 gctacagact cgatgatccg gatgaagcga accagaacta cttagcagat gaagaggagg 181 aagcagaaga agaggctcgg gtgacggtgg tgcccaaatc ggaggaggag gaagaagagg 241 aggagaaaga agaggaggaa gaggaggaaa aggaggagga agagggtcaa ggtcagccaa 301 caggcaatgc ctggtggcag aaattgcaga tcatgagtga atacctgtgg gatccagaga 361 gaaggatgtt tctggcccga acaggtcaga gttggagcct gatcttactc atttacttct 421 tcttctatgc ctccttggct gctgtgatca ccctctgcat gtacacacta tttctgacca 481 tcagtcccta tataccaacc ttcacggagc gggtaaagcc tcctggagtt atgatcagac 541 ccttcgccca tagccttaac ttcaacttca acgtttctga acccgacact tggcagcatt 601 atgtgattag cctaaatggc tttctccagg gttataatga cagtcttcaa gaggaaatga 661 atgtagattg tcccccgggg cagtacttca tccaagatgg caatgaggat gaggacaaga 721 aggcctgcca atttaagcgc tccttcctaa agaactgctc tggtctggag gacccaactt 781 ttggatactc tactggacag ccctgcatcc ttctaaagat gaaccggatt gtaggctttc 841 gtcctgagct tggagatcct gtgaaggttt cctgcaaagt tcagagaggt gatgaaaatg 901 acatccgatc catcagttac tacccagagt cggcttcttt tgacctccgc tactaccctt 961 actacggcaa actgactcac gttaactaca catccccctt ggtggcaatg cactttacag 1021 acgtggtgaa gaaccaagca gtgcctgtgc agtgccaact gaagggcaaa ggcgtcataa 1081 atgatgtcat caatgatcgt tttgtgggca gggtaatctt taccctgaac atagaaactt 1141 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1201 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1261 ggatta