Construct: ORF ccsbBroad304_02796
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009321.1_s304c1, BRDN0000461299
- Derived from:
- ccsbBroadEn_02796
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHST5 (23563)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02796
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23563 | CHST5 | carbohydrate sulfotransfera... | NM_024533.5 | 100% | 100% | |
| 2 | human | 4166 | CHST6 | carbohydrate sulfotransfera... | NM_021615.5 | 82.1% | 79.8% | (many diffs) |
| 3 | human | 4166 | CHST6 | carbohydrate sulfotransfera... | XM_005255955.5 | 82.1% | 79.8% | (many diffs) |
| 4 | human | 4166 | CHST6 | carbohydrate sulfotransfera... | XM_011523085.3 | 82.1% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1302
- ORF length:
- 1233
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcatgagg gcccgggtcc ctaaagttgc ccactccacc cgaaggcccc 121 cagccgcccg catgtggctg ccacggttct ccagcaagac agtgacagtg ctcctcctgg 181 cacagaccac ctgcctcctg ctcttcatca tctcccggcc agggccctca tccccagccg 241 gcggcgagga tcgtgtgcac gtgctggtgc tgtcctcgtg gcgctcgggc tcatccttct 301 tgggccagct cttcagccag caccccgacg tcttctacct gatggagccc gcgtggcatg 361 tgtggaccac cctgtcgcag ggcagcgcgg caacgctgca catggccgtg cgcgacctga 421 tgcgctctat ctttttgtgc gacatggacg tgtttgatgc ctacatgcca cagagccgaa 481 acctgtccgc ctttttcaac tgggcaacga gccgcgcgct gtgctcgccg cccgcctgca 541 gcgcctttcc ccgaggcacc atcagcaagc aggacgtatg caagacactg tgcacgcggc 601 agccattcag cctggcccgg gaggcctgcc gctcctacag ccacgtggtg ctcaaggagg 661 tgcgcttctt caacctgcag gtgctctacc cgctgctcag cgaccccgcg ctcaacctgc 721 gcatcgtgca cctggtgcgc gacccgcggg ccgtgctgcg ctcccgggag gcggcgggcc 781 cgatactggc acgcgacaac ggcatcgtgc tgggcaccaa cggcaagtgg gtggaggccg 841 accctcacct gcgcctgatt cgcgaggtgt gccgcagcca cgtgcgcatc gccgaggccg 901 ccacactcaa gccgccaccc ttcctgcgcg gccgctaccg cctggtgcgc ttcgaggacc 961 tggcgcggga gccgctggca gagatccgcg cactctacgc cttcaccggc ctgaccctca 1021 cgccacagct cgaggcctgg atccacaaca tcacccacgg gtcggggatc ggcaagccaa 1081 tcgaggcctt ccatacttcg tctaggaatg cgcgcaacgt ctcccaggcc tggcgccacg 1141 cgttgccctt cactaagatc ctgcgcgtgc aggaggtgtg cgccggcgcg ctgcagctgc 1201 tgggctaccg gcctgtgtac tctgcggacc agcagcgtga cctcaccctg gatctggtgc 1261 tgccacgagg cccagaccac ttcagctggg catcgcctga cttgccaact ttcttgtaca 1321 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1381 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta