Construct: ORF ccsbBroad304_02825
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000242.1_s304c1, BRDN0000402462
- Derived from:
- ccsbBroadEn_02825
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLA2 (23649)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02825
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23649 | POLA2 | DNA polymerase alpha 2, acc... | NM_002689.4 | 100% | 100% | |
| 2 | human | 23649 | POLA2 | DNA polymerase alpha 2, acc... | XM_011544877.3 | 93.5% | 92.2% | (many diffs) |
| 3 | human | 23649 | POLA2 | DNA polymerase alpha 2, acc... | XM_017017452.2 | 93.3% | 92.3% | (many diffs) |
| 4 | human | 23649 | POLA2 | DNA polymerase alpha 2, acc... | XM_011544878.3 | 92.2% | 91.8% | (many diffs) |
| 5 | human | 23649 | POLA2 | DNA polymerase alpha 2, acc... | XM_011544879.2 | 86.9% | 85.1% | 1518_1519ins70;1560_1561ins164 |
| 6 | human | 23649 | POLA2 | DNA polymerase alpha 2, acc... | XM_011544881.3 | 86.9% | 85.1% | 1518_1519ins70;1560_1561ins164 |
| 7 | mouse | 18969 | Pola2 | polymerase (DNA directed), ... | XM_006531684.3 | 38.2% | 39.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1860
- ORF length:
- 1794
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgcatccgcc cagcagctgg cggaggagct gcagatcttc ggcctagact 121 gcgaggaggc tctaattgag aaattggtag agctttgtgt tcagtatgga cagaatgagg 181 agggaatggt aggcgagctt atagccttct gcaccagcac acataaagtt ggccttacct 241 cagagatcct gaactctttt gagcatgagt ttctgagcaa aagattatcg aaagccaggc 301 atagtacctg caaggacagt ggccatgcag gagctagaga cattgtttcc attcaagagc 361 taattgaagt ggaagaagaa gaggaaatcc tcttgaactc ttacaccaca ccttcaaagg 421 gttctcagaa gcgagctatc tctaccccag aaacccccct aacaaaaagg agtgtgtcaa 481 ctcgtagccc ccatcagcta ctctcaccgt caagtttctc tccaagtgct actccctccc 541 agaaatacaa ctcacgaagt aaccgaggag aagtggttac ctccttcggc ttagcacagg 601 gagtatcttg gtctgggaga ggaggagctg gaaacatcag cctgaaggtc ttgggatgtc 661 cagaggcact aactgggagc tacaaatcca tgtttcagaa gctcccagac attcgagaag 721 ttctgacctg taagatagaa gaacttggca gcgaactcaa ggaacattac aagattgaag 781 ctttcactcc tttgctagcc ccagcacagg agcctgtcac tctgctgggc cagattggct 841 gtgatagcaa cgggaagctg aacaacaagt cagtgattct cgagggagac cgggaacatt 901 cctcgggtgc tcaaattcca gtggatttat ctgagcttaa ggaatattct ctgtttcctg 961 gacaggttgt aattatggaa ggaatcaaca ccactggtag gaaacttgtt gccaccaaac 1021 tctacgaggg tgtgccactt ccattttatc agcccactga agaggatgca gactttgagc 1081 aaagcatggt cctggttgcc tgtggaccat acaccacatc tgacagcatc acgtatgacc 1141 ccctgcttga cctgattgct gtcatcaacc atgaccggcc agatgtctgc atcctgtttg 1201 gccctttcct ggatgctaag catgaacagg tggagaattg tctactgaca agtccatttg 1261 aagacatttt caagcagtgt ctacgaacaa ttattgaagg cacaagaagc tccggctccc 1321 accttgtctt tgtcccgtca ttgagagatg tgcaccatga gcctgtgtac ccccagccgc 1381 ctttcagcta ctccgatctg tctcgagagg acaaaaagca agtacagttt gtgtccgagc 1441 cctgcagcct ctccataaac ggagtgatct tcggcttgac atccacagat ctgcttttcc 1501 acctgggggc cgaggagatc agtagttctt ccggaacttc agacagattc agccgaatac 1561 tcaagcacat cttgacccag aggagctact acccactcta cccgccccaa gaagacatgg 1621 ccattgacta tgagtcgttc tatgtttacg cacagctgcc tgtcacccca gatgtcctca 1681 tcatcccgtc agagctgagg tacttcgtga aggatgtcct cggctgtgtc tgtgtgaacc 1741 ctgggcgcct taccaaaggg caggtgggag gcaccttcgc ccgactctac cttaggaggc 1801 cggcagcgga cggggcagag aggcagagcc catgcattgc tgtgcaggtc gtcaggatct 1861 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1921 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1981 ggatta