Construct: ORF ccsbBroad304_02909

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015285.1_s304c1, BRDN0000405663
Derived from:
ccsbBroadEn_02909
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HACL1 (26061)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02909
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26061 HACL1 2-hydroxyacyl-CoA lyase 1 NM_012260.4 100% 100%
2 human 26061 HACL1 2-hydroxyacyl-CoA lyase 1 NM_001284413.2 95.3% 95.3% 226_227ins81
3 human 26061 HACL1 2-hydroxyacyl-CoA lyase 1 NM_001284415.2 89.6% 89.6% 379_380ins78;913_914ins102
4 human 26061 HACL1 2-hydroxyacyl-CoA lyase 1 NM_001284416.2 85.8% 85.8% 308_309ins246
5 human 26061 HACL1 2-hydroxyacyl-CoA lyase 1 NR_104315.2 77.4% (many diffs)
6 mouse 56794 Hacl1 2-hydroxyacyl-CoA lyase 1 NM_019975.3 85.5% 87.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1800
ORF length:
1734
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc ggacagtaac ttcgcagagc gcagcgagga gcaggtgtct ggtgctaaag
121tcatcgctca ggccctgaaa acgcaagatg tggagtacat atttggcatc gtaggcatcc
181cagtgaccga aatcgccatt gctgcccagc agctaggcat caagtacatc gggatgagga
241atgagcaagc ggcttgttat gctgcctccg cgattggata tctgacaagc aggccaggag
301tctgccttgt tgtttctggc ccaggtctca tccatgcctt gggcggtatg gcaaatgcaa
361acatgaactg ctggcccttg cttgtgattg gtggttcctc tgaaagaaac caagaaacaa
421tgggagcttt ccaggagttt cctcaggttg aagcttgtag attatatacc aagttctctg
481cccgcccaag cagcatagaa gctattcctt ttgttattga aaaggcagtg agaagcagta
541tctatggtcg tccaggtgct tgctatgttg acataccagc agattttgtg aaccttcagg
601tgaatgtgaa ttctataaag tacatggaac gctgcatgtc acctcctatt agcatggcag
661aaacctctgc tgtgtgcacg gcggcttctg ttattaggaa tgccaaacaa ccccttctta
721tcatcgggaa aggtgctgct tacgctcatg cagaagagag tatcaagaaa ttggtggagc
781aatataaact gccatttttg cccaccccta tggggaaggg tgttgtccct gacaaccatc
841catactgtgt aggtgcagcc agatccaggg ctttgcaatt tgctgatgta attgtgttat
901ttggtgccag actaaattgg attttacatt ttggactgcc tccaagatat cagccagatg
961tgaagtttat ccaggttgat atctgtgcag aagaattggg gaataatgta aagcccgctg
1021ttactttgct aggaaacata catgctgtca ctaagcagct tttagaggaa cttgataaaa
1081caccatggca gtatcctcca gagagcaagt ggtggaaaac tctgagagaa aaaatgaaga
1141gcaatgaagc tgcatccaag gaactagctt ctaaaaaatc cctgcctatg aattattaca
1201cagtattcta ccatgttcaa gaacaactac ctagagactg tttcgtggta agtgaaggag
1261caaatactat ggacattgga cggactgtgc ttcagaacta ccttcctcgt cacaggcttg
1321atgctggtac tttcggaaca atgggagttg gtttgggatt tgctattgca gctgccgtgg
1381tggctaaaga tagaagccct gggcaatgga tcatctgtgt ggaaggagac agtgcatttg
1441ggttttctgg catggaggta gaaaccatct gcaggtacaa cttgccaatc atactgttgg
1501tagtgaataa caatggaatt taccaaggtt ttgatacaga tacttggaaa gaaatgttaa
1561aatttcaaga tgctactgca gtggtccctc caatgtgttt gctgccaaat tcacattatg
1621agcaagtcat gactgcattt ggaggcaaag ggtattttgt acaaacacca gaagaactcc
1681aaaaatccct gaggcagagc ctagcagaca caactaaacc ttctcttatc aacatcatga
1741ttgagccaca agccacacgg aaggcccagg attttcattg gctgacccgc tctaatatgt
1801acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1861attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1921ggatta

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