Construct: ORF ccsbBroad304_02955
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001948.1_s304c1, BRDN0000410476
- Derived from:
- ccsbBroadEn_02955
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYCBP (26292)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02955
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26292 | MYCBP | MYC binding protein | NM_012333.5 | 100% | 100% | |
| 2 | human | 100527950 | GJA9-MYCBP | GJA9-MYCBP readthrough | NR_037635.1 | 11.4% | (many diffs) | |
| 3 | human | 100527950 | GJA9-MYCBP | GJA9-MYCBP readthrough | NR_037636.1 | 10.5% | (many diffs) | |
| 4 | human | 26292 | MYCBP | MYC binding protein | NR_037632.1 | 9.6% | 1_73del;160_161ins59;324_2524del | |
| 5 | human | 100527950 | GJA9-MYCBP | GJA9-MYCBP readthrough | NR_037634.1 | 6.9% | (many diffs) | |
| 6 | human | 100527950 | GJA9-MYCBP | GJA9-MYCBP readthrough | NR_037633.1 | 5.8% | (many diffs) | |
| 7 | mouse | 56309 | Mycbp | MYC binding protein | NM_019660.3 | 95.1% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 375
- ORF length:
- 309
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ccattacaaa gccgccgact cgaagcgtga gcagttccgg aggtacttgg 121 agaagtcggg ggtgctggac acgctgacca aggtgttggt agccttatat gaagaaccag 181 agaaacctaa cagtgctttg gattttttaa agcatcactt aggagctgct actccagaaa 241 atccagaaat agagctgctt cgcctagaac tggccgaaat gaaagagaag tatgaagcta 301 ttgtagaaga aaataaaaaa ctgaaagcaa agcttgctca gtatgaacca cctcaggagg 361 agaagcgtgc tgaataccca actttcttgt acaaagtggt tggtaagcct atccctaacc 421 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 481 cgacaatcaa cctctggatt a