Construct: ORF ccsbBroad304_02956
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000350.1_s304c1, BRDN0000400580
- Derived from:
- ccsbBroadEn_02956
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EHF (26298)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02956
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26298 | EHF | ETS homologous factor | NM_012153.6 | 100% | 100% | |
| 2 | human | 26298 | EHF | ETS homologous factor | XM_005252860.1 | 100% | 100% | |
| 3 | human | 26298 | EHF | ETS homologous factor | XM_011519984.1 | 100% | 100% | |
| 4 | human | 26298 | EHF | ETS homologous factor | XM_011519985.1 | 100% | 100% | |
| 5 | human | 26298 | EHF | ETS homologous factor | XM_024448431.1 | 100% | 100% | |
| 6 | human | 26298 | EHF | ETS homologous factor | XM_024448432.1 | 100% | 100% | |
| 7 | human | 26298 | EHF | ETS homologous factor | XM_024448433.1 | 100% | 100% | |
| 8 | human | 26298 | EHF | ETS homologous factor | XM_024448434.1 | 100% | 100% | |
| 9 | human | 26298 | EHF | ETS homologous factor | XM_011519983.1 | 99% | 99% | 1_9delATGGAGATC |
| 10 | human | 26298 | EHF | ETS homologous factor | NM_001206616.2 | 93.1% | 93.1% | 1_66del |
| 11 | human | 26298 | EHF | ETS homologous factor | XM_005252857.1 | 92.8% | 92.8% | 1_66del;609_610insGCA |
| 12 | human | 26298 | EHF | ETS homologous factor | NM_001206615.2 | 92.3% | 92.3% | 474_475ins69 |
| 13 | human | 26298 | EHF | ETS homologous factor | XM_005252862.1 | 92.3% | 92.3% | 474_475ins69 |
| 14 | human | 26298 | EHF | ETS homologous factor | XM_005252861.1 | 86% | 86% | 1_66del;540_541ins69 |
| 15 | mouse | 13661 | Ehf | ets homologous factor | NM_007914.3 | 89% | 93.6% | (many diffs) |
| 16 | mouse | 13661 | Ehf | ets homologous factor | XM_006498697.2 | 89% | 93.6% | (many diffs) |
| 17 | mouse | 13661 | Ehf | ets homologous factor | XM_006498698.3 | 89% | 93.6% | (many diffs) |
| 18 | mouse | 13661 | Ehf | ets homologous factor | XM_006498699.1 | 89% | 93.6% | (many diffs) |
| 19 | mouse | 13661 | Ehf | ets homologous factor | XM_006498700.3 | 89% | 93.6% | (many diffs) |
| 20 | mouse | 13661 | Ehf | ets homologous factor | XM_006498701.3 | 89% | 93.6% | (many diffs) |
| 21 | mouse | 13661 | Ehf | ets homologous factor | XM_006498702.3 | 89% | 93.6% | (many diffs) |
| 22 | mouse | 13661 | Ehf | ets homologous factor | XM_006498703.1 | 88.6% | 93.3% | (many diffs) |
| 23 | mouse | 13661 | Ehf | ets homologous factor | XM_006498695.3 | 85.8% | 90.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 966
- ORF length:
- 900
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat tctggaagga ggtggtgtaa tgaatctcaa ccccggcaac aacctccttc 121 accagccgcc agcctggaca gacagctact ccacgtgcaa tgtttccagt gggttttttg 181 gaggccagtg gcatgaaatt catcctcagt actggaccaa gtaccaggtg tgggagtggc 241 tccagcacct cctggacacc aaccagctgg atgccaattg tatccctttc caagagttcg 301 acatcaacgg cgagcacctc tgcagcatga gtttgcagga gttcacccgg gcggcaggga 361 cggcggggca gctcctctac agcaacttgc agcatctgaa gtggaacggc cagtgcagta 421 gtgacctgtt ccagtccaca cacaatgtca ttgtcaagac tgaacaaact gagccttcca 481 tcatgaacac ctggaaagac gagaactatt tatatgacac caactatggt agcacagtag 541 atttgttgga cagcaaaact ttctgccggg ctcagatctc catgacaacc accagtcacc 601 ttcctgttgc agagtcacct gatatgaaaa aggagcaaga cccccctgcc aagtgccaca 661 ccaaaaagca caacccgaga gggactcact tatgggaatt catccgcgac atcctcttga 721 acccagacaa gaacccagga ttaataaaat gggaagaccg atctgagggc gtcttcaggt 781 tcttgaaatc agaggcagtg gctcagctat ggggtaaaaa gaagaacaac agcagcatga 841 cctatgaaaa gctcagccga gctatgagat attactacaa aagagaaatt ctggagcgtg 901 tggatggacg aagactggta tataaatttg ggaagaatgc ccgaggatgg agagaaaatg 961 aaaactgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1021 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1081 acctctggat ta