Construct: ORF ccsbBroad304_02986

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006488.1_s304c1, BRDN0000399304
Derived from:
ccsbBroadEn_02986
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NSG1 (27065)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02986
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27065 NSG1 neuronal vesicle traffickin... NM_001040101.1 100% 100%
2 human 27065 NSG1 neuronal vesicle traffickin... NM_001287763.1 100% 100%
3 human 27065 NSG1 neuronal vesicle traffickin... NM_014392.4 100% 100%
4 human 27065 NSG1 neuronal vesicle traffickin... NM_001287764.1 85.4% 85.4% 0_1ins81
5 mouse 18196 Nsg1 neuron specific gene family... NM_010942.3 93.1% 98.3% (many diffs)
6 mouse 18196 Nsg1 neuron specific gene family... XM_006503773.1 93.1% 98.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
621
ORF length:
555
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt gaagttgggg aacaatttcg cagagaaggg caccaagcag ccgctgctgg
121aggatggctt cgacaccatt cccctgatga cgcccctcga tgtcaatcag ctgcagttcc
181cgcccccgga taaggtggtc gtgaaaacta agaccgagta tgaacctgac cgcaagaaag
241ggaaagcacg tcctccccaa attgctgagt tcaccgtcag catcacggag ggtgtcaccg
301agaggtttaa ggtctccgtg ttggtcctct tcgccctggc cttcctcacc tgcgtcgtct
361tcctggttgt ctacaaggtg tacaagtatg accgcgcctg ccccgatggg ttcgtcctca
421agaacaccca gtgcatccca gaaggcttgg agagctacta cgcggagcaa gactccagtg
481cccgggagaa attttacaca gtcataaacc actacaacct ggccaagcag agcatcacgc
541gctccgtatc gccctggatg tcagttctgt cagaagagaa gctgtccgag caggagactg
601aagcggctga gaagtcagct tacccaactt tcttgtacaa agtggttggt aagcctatcc
661ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
721ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)