Construct: ORF ccsbBroad304_03027
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004182.1_s304c1, BRDN0000402797
- Derived from:
- ccsbBroadEn_03027
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB30 (27314)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03027
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27314 | RAB30 | RAB30, member RAS oncogene ... | NM_001286059.2 | 100% | 100% | |
| 2 | human | 27314 | RAB30 | RAB30, member RAS oncogene ... | NM_001286060.2 | 100% | 100% | |
| 3 | human | 27314 | RAB30 | RAB30, member RAS oncogene ... | NM_001286061.1 | 100% | 100% | |
| 4 | human | 27314 | RAB30 | RAB30, member RAS oncogene ... | NM_014488.5 | 100% | 100% | |
| 5 | mouse | 75985 | Rab30 | RAB30, member RAS oncogene ... | NM_029494.2 | 94% | 100% | (many diffs) |
| 6 | mouse | 75985 | Rab30 | RAB30, member RAS oncogene ... | XM_006508293.3 | 94% | 100% | (many diffs) |
| 7 | mouse | 75985 | Rab30 | RAB30, member RAS oncogene ... | XM_006508294.2 | 94% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 675
- ORF length:
- 609
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tatggaagat tatgatttcc tgttcaaaat tgttttaatt ggcaacgctg 121 gtgtggggaa gacgtgcctc gtccgaagat tcactcaggg tcttttcccc ccaggtcaag 181 gagccacaat tggagttgat tttatgatta agacagtgga gattaatggt gaaaaagtaa 241 agctacagat ctgggacaca gcaggtcaag agagatttcg gtccattacc cagagttact 301 accgaagcgc caatgccttg atcctcacct atgacattac ctgtgaggaa tccttccgtt 361 gccttcctga gtggctgcgg gagatagaac aatatgccag caacaaggtc atcactgtgt 421 tagtgggcaa caagattgac ctggctgaaa ggagagaggt ttcccagcag cgagctgaag 481 aattctcaga agctcaggac atgtattatc tggagacctc agccaaggaa tctgataatg 541 tggagaaact cttccttgac ttagcatgcc gactcatcag tgaagccaga cagaacacac 601 ttgtgaacaa tgtatcctca cccttacctg gagaagggaa aagcatcagc tatttgactt 661 gttgtaattt caactaccca actttcttgt acaaagtggt tggtaagcct atccctaacc 721 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 781 cgacaatcaa cctctggatt a