Construct: ORF ccsbBroad304_03032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010476.1_s304c1, BRDN0000410393
- Derived from:
- ccsbBroadEn_03032
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTATSF1 (27336)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03032
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27336 | HTATSF1 | HIV-1 Tat specific factor 1 | NM_001163280.1 | 100% | 100% | |
| 2 | human | 27336 | HTATSF1 | HIV-1 Tat specific factor 1 | NM_014500.5 | 100% | 100% | |
| 3 | human | 27336 | HTATSF1 | HIV-1 Tat specific factor 1 | XM_005262404.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2331
- ORF length:
- 2265
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cggcaccaac ttggatggga acgatgagtt tgatgagcag ttgcgaatgc 121 aagaattgta cggagacggc aaggatggtg acacccagac cgatgccggc ggagaacccg 181 attctctcgg gcagcagccg acggacactc cctacgagtg ggacctggac aaaaaggctt 241 ggttccccaa gattactgaa gatttcattg ctacatatca ggccaattat ggcttctcta 301 acgatggcgc atctagttct accgcaaatg ttgaagatgt ccatgctagg actgcagagg 361 aacctccaca agaaaaagcc ccggaaccca ctgatgccag aaagaaggga gaaaaaagaa 421 aggctgagtc aggatggttt catgttgaag aagacagaaa tacaaatgta tacgtgtctg 481 gtttgcctcc agatattaca gtggatgaat ttatacaact tatgtccaag tttggcatta 541 ttatgagaga tcctcagaca gaagaattta aggtcaaact ttacaaagat aatcaaggaa 601 atcttaaagg agacggtctt tgctgttatt tgaaaagaga atctgtggaa cttgcattaa 661 aacttttgga tgaagatgaa attagaggct acaaattaca tgttgaggtg gcaaagtttc 721 aactgaaggg agaatatgat gcctcaaaga agaagaagaa gtgcaaagac tataagaaga 781 agctgtctat gcaacaaaag cagttggatt ggagacctga gaggcgagcc ggaccatccc 841 ggatgcgcca tgagcgagtt gtcatcatca agaatatgtt tcatcctatg gattttgagg 901 atgatccgtt ggtgctgaat gagatcagag aagaccttcg agtagagtgt tcgaagtttg 961 gacaaattag gaaactcctt ctctttgata ggcacccaga tggtgtggcc tctgtgtcct 1021 ttcgggatcc agaggaagct gattattgta ttcagactct cgatggaaga tggtttggtg 1081 gccgtcaaat cactgcccag gcatgggatg ggactacaga ttatcaggtg gaggaaacct 1141 caagagaaag ggaggaaagg ctgagaggat gggaggcttt cctcaatgct cctgaggcca 1201 acagaggcct taggcgttca gattctgtct ctgcttccga aagggcaggg ccttctagag 1261 caaggcattt ttcagagcac cccagcacat ctaaaatgaa tgctcaagaa actgcaactg 1321 gaatggcgtt tgaagaacct atagatgaga agaagtttga aaagacagaa gatgggggag 1381 aatttgaaga aggtgcttct gaaaacaatg ctaaggaaag tagccccgaa aaagaggctg 1441 aagaaggctg ccctgaaaaa gaatctgaag agggctgccc caaaagaggg tttgaaggca 1501 gctgctccca aaaagagtct gaagaaggca atcccgtaag aggatctgaa gaggatagtc 1561 ctaaaaaaga gtctaaaaag aagacactca aaaatgattg tgaagagaat ggccttgcaa 1621 aggaatctga agatgacctc aacaaggagt ctgaagagga ggttggcccc acaaaagagt 1681 ccgaagaaga tgactcagag aaagagtctg atgaagactg ctctgaaaaa cagtctgaag 1741 atggctccga aagagaattt gaagaaaatg gtctcgagaa agatttggac gaggaaggtt 1801 ctgaaaagga gcttcatgaa aatgttcttg acaaagagtt agaagaaaat gactctgaaa 1861 actccgaatt tgaagatgac ggctctgaaa aagtgttaga tgaggaaggc tctgagagag 1921 agtttgacga agattcagat gaaaaggaag aagaggagga tacatatgaa aaagtatttg 1981 atgatgagtc tgatgagaaa gaggatgaag aatatgcaga tgaaaagggg cttgaagctg 2041 ctgataaaaa ggcggaagaa ggtgatgcag atgaaaagct gtttgaagag tcagatgaca 2101 aggaagatga agatgcagat ggaaaggaag ttgaagatgc tgacgaaaag ttgttcgaag 2161 atgatgattc caatgagaag ttgtttgatg aggaggaaga ttccagtgag aagttgtttg 2221 acgattctga tgagaggggg actttgggtg gttttgggag tgttgaagaa gggcccctat 2281 ccactggcag cagctttatt ctcagtagcg atgatgatga cgatgatatt tacccaactt 2341 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 2401 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta