Construct: ORF ccsbBroad304_03053
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000574.1_s304c1, BRDN0000403355
- Derived from:
- ccsbBroadEn_03053
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM14A (28978)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03053
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28978 | TMEM14A | transmembrane protein 14A | NM_014051.4 | 100% | 100% | |
| 2 | human | 28978 | TMEM14A | transmembrane protein 14A | XM_024446409.1 | 100% | 100% | |
| 3 | mouse | 75712 | Tmem14a | transmembrane protein 14A | NM_029398.3 | 89.5% | 92.9% | (many diffs) |
| 4 | mouse | 75712 | Tmem14a | transmembrane protein 14A | XM_006495581.2 | 83.6% | 86.7% | (many diffs) |
| 5 | mouse | 75712 | Tmem14a | transmembrane protein 14A | NM_001290679.1 | 58.9% | 52.9% | (many diffs) |
| 6 | mouse | 75712 | Tmem14a | transmembrane protein 14A | XM_006495579.2 | 56.2% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 363
- ORF length:
- 297
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cctgatcggt tttggttatg cagccctcgt gacatttgga agcatttttg 121 gatataagcg gagaggtggt gttccgtctt tgattgctgg tctttttgtt ggatgtttgg 181 ccggctatgg agcttaccgt gtctccaatg acaaacgaga tgtaaaagtg tcactgttta 241 cagctttctt cctggctacc ataatgggtg tgagatttaa gaggtccaag aaaataatgc 301 ctgctggttt ggttgcaggt ttaagcctca tgatgatcct gagacttgtc ttgttgctgc 361 tctgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 421 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 481 tctggatta