Construct: ORF ccsbBroad304_03093
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002905.1_s304c1, BRDN0000460554
- Derived from:
- ccsbBroadEn_03093
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C2orf27A (29798)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03093
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29798 | C2orf27A | chromosome 2 open reading f... | NM_001354871.2 | 100% | 100% | |
| 2 | human | 29798 | C2orf27A | chromosome 2 open reading f... | NM_013310.5 | 100% | 100% | |
| 3 | human | 29798 | C2orf27A | chromosome 2 open reading f... | NM_001354872.2 | 66.5% | 66.5% | 0_1ins204 |
| 4 | human | 408029 | C2orf27B | chromosome 2 open reading f... | NR_161366.1 | 45.2% | (many diffs) | |
| 5 | human | 29798 | C2orf27A | chromosome 2 open reading f... | NR_149013.2 | 26.3% | 1_1314del;1343_1522del;2104_2308del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 678
- ORF length:
- 609
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gacagtcaag tggaaacaac tgtctgctcc tgcttcaggt gcagaaatac 121 agcgatttcc agtgccagct gttgagccag tgccagcacc aggggcagat tcccctccag 181 ggacagcgct ggagctagag gaagctccag agccctcctg ccgctgccct gggactgccc 241 aggaccagcc cagtgaggag ctgcctgact tcatggcacc tcctgtagag ccaccggcct 301 cagccctgga gctgaaagtg tggctggagc tagaggtggc agagaggggt ggccagcaca 361 gctccagcca gcagctccca cactgctccc agtcctgggc acagtggaag ctatggaggc 421 agagaccagg gtttgcaatc tgggctcctc tgcctcactg gagagggact tctctcattc 481 agcagagcag cagccctgct gctgaagggc ctgctgctac tgctgctggg gctgtttgcc 541 tgcctgcagg aggtgctgga gagcaagaaa aggagcctgt gagcaggggt tccagcaggt 601 cctcctgctc ccagaggcga cctcctcctc caggcatgga ggtttgccct cagctgggca 661 tctgggccat ttgccccttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 721 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 781 agtcgacaat caacctctgg atta