Construct: ORF ccsbBroad304_03135
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006275.1_s304c1, BRDN0000461559
- Derived from:
- ccsbBroadEn_03135
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERVW-1 (30816)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03135
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30816 | ERVW-1 | endogenous retrovirus group... | NM_001130925.2 | 100% | 100% | |
| 2 | human | 30816 | ERVW-1 | endogenous retrovirus group... | NM_014590.3 | 100% | 100% | |
| 3 | human | 105377310 | LOC105377310 | syncytin-1-like | XM_011532442.2 | 38.5% | 34.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1683
- ORF length:
- 1614
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccctccct tatcatattt ttctctttac tgttctttta ccctctttca 121 ctctcactgc accccctcca tgccgctgta tgaccagtag ctccccttac caagagtttc 181 tatggagaat gcagcgtccc ggaaatattg atgccccatc gtataggagt ctttctaagg 241 gaacccccac cttcactgcc cacacccata tgccccgcaa ctgctatcac tctgccactc 301 tttgcatgca tgcaaatact cattattgga caggaaaaat gattaatcct agttgtcctg 361 gaggacttgg agtcactgtc tgttggactt acttcaccca aactggtatg tctgatgggg 421 gtggagttca agatcaggca agagaaaaac atgtaaaaga agtaatctcc caactcaccc 481 gggtacatgg cacctctagc ccctacaaag gactagatct ctcaaaacta catgaaaccc 541 tccgtaccca tactcgcctg gtaagcctat ttaataccac cctcactggg ctccatgagg 601 tctcggccca aaaccctact aactgttgga tatgcctccc cctgaacttc aggccatatg 661 tttcaatccc tgtacctgaa caatggaaca acttcagcac agaaataaac accacttccg 721 ttttagtagg acctcttgtt tccaatctgg aaataaccca tacctcaaac ctcacctgtg 781 taaaatttag caatactaca tacacaacca actcccaatg catcaggtgg gtaactcctc 841 ccacacaaat agtctgccta ccctcaggaa tattttttgt ctgtggtacc tcagcctatc 901 gttgtttgaa tggctcttca gaatctatgt gcttcctctc attcttagtg ccccctatga 961 ccatctacac tgaacaagat ttatacagtt atgtcatatc taagccccgc aacaaaagag 1021 tacccattct tccttttgtt ataggagcag gagtgctagg tgcactaggt actggcattg 1081 gcggtatcac aacctctact cagttctact acaaactatc tcaagaacta aatggggaca 1141 tggaacgggt cgccgactcc ctggtcacct tgcaagatca acttaactcc ctagcagcag 1201 tagtccttca aaatcgaaga gctttagact tgctaaccgc tgaaagaggg ggaacctgtt 1261 tatttttagg ggaagaatgc tgttattatg ttaatcaatc cggaatcgtc actgagaaag 1321 ttaaagaaat tcgagatcga atacaacgta gagcagagga gcttcgaaac actggaccct 1381 ggggcctcct cagccaatgg atgccctgga ttctcccctt cttaggacct ctagcagcta 1441 taatattgct actcctcttt ggaccctgta tctttaacct ccttgttaac tttgtctctt 1501 ccagaatcga agctgtaaaa ctacaaatgg agcccaagat gcagtccaag actaagatct 1561 accgcagacc cctggaccgg cctgctagcc cacgatctga tgttaatgac atcaaaggca 1621 cccctcctga ggaaatctca gctgcacaac ctctactacg ccccaattca gcaggaagca 1681 gtttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1741 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1801 tctggatta