Construct: ORF ccsbBroad304_03514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000218.1_s304c1, BRDN0000398861
- Derived from:
- ccsbBroadEn_03514
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM248 (55069)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03514
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55069 | TMEM248 | transmembrane protein 248 | NM_017994.5 | 100% | 100% | |
| 2 | human | 55069 | TMEM248 | transmembrane protein 248 | XM_005250482.4 | 100% | 100% | |
| 3 | human | 55069 | TMEM248 | transmembrane protein 248 | XM_024446820.1 | 100% | 100% | |
| 4 | human | 55069 | TMEM248 | transmembrane protein 248 | XM_024446821.1 | 100% | 100% | |
| 5 | human | 55069 | TMEM248 | transmembrane protein 248 | XM_024446822.1 | 100% | 100% | |
| 6 | human | 55069 | TMEM248 | transmembrane protein 248 | XM_024446819.1 | 97.5% | 97.5% | 1_24del |
| 7 | mouse | 71667 | Tmem248 | transmembrane protein 248 | NM_001081394.1 | 86.7% | 94.5% | (many diffs) |
| 8 | mouse | 71667 | Tmem248 | transmembrane protein 248 | NM_027854.1 | 86.7% | 94.5% | (many diffs) |
| 9 | mouse | 71667 | Tmem248 | transmembrane protein 248 | XM_011240907.2 | 86.7% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1008
- ORF length:
- 942
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt cagcatcaac cccctggaga acctgaaggt gtacatcagc agtcggcctc 121 ccctggtggt cttcatgatc agcgtaagcg ccatggccat agctttcctg accctgggct 181 acttcttcaa aatcaaggag attaaatccc cagaaatggc agaggattgg aatacttttc 241 tgctacggtt caatgatttg gacttgtgtg tatcagagaa tgaaaccctc aagcatctca 301 caaacgacac cacaactccg gaaagtacaa tgaccagcgg gcaggcccga gcttccaccc 361 agtcccccca ggccctggag gactcgggcc cggtgaatat ctcagtctca atcaccctaa 421 ccctggaccc actgaaaccc ttcggagggt attcccgcaa cgtcacccat ctgtactcaa 481 ccatcttagg gcatcagatt ggactttcag gcagggaagc ccacgaggag ataaacatca 541 ccttcaccct gcctacagcg tggagctcag atgactgcgc cctccacggt cactgtgagc 601 aggtggtatt cacagcctgc atgaccctca cggccagccc tggggtgttc cccgtcactg 661 tacagccacc gcactgtgtt cctgacacgt acagcaacgc cacgctctgg tacaagatct 721 tcacaactgc cagagatgcc aacacaaaat acgcccaaga ttacaatcct ttctggtgtt 781 ataagggggc cattggaaaa gtctatcatg ctttaaatcc caagcttaca gtgattgttc 841 cagatgatga ccgttcatta ataaatttgc atctcatgca caccagttac ttcctctttg 901 tgatggtgat aacaatgttt tgctatgctg ttatcaaggg cagacctagc aaattgcgtc 961 agagcaatcc tgaattttgt cccgagaagg tggctttggc tgaagcctac cccactttct 1021 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1081 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta