Construct: ORF ccsbBroad304_03632

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018209.1_s304c1, BRDN0000408763
Derived from:
ccsbBroadEn_03632
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VAC14 (55697)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03632
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55697 VAC14 VAC14 component of PIKFYVE ... NM_018052.5 100% 100%
2 human 55697 VAC14 VAC14 component of PIKFYVE ... XM_005256038.4 94.3% 91.6% (many diffs)
3 human 55697 VAC14 VAC14 component of PIKFYVE ... XM_011523224.3 79.7% 70.1% (many diffs)
4 human 55697 VAC14 VAC14 component of PIKFYVE ... XM_011523223.3 79.3% 70.8% (many diffs)
5 human 55697 VAC14 VAC14 component of PIKFYVE ... XM_005256041.4 79.2% 72.5% (many diffs)
6 human 55697 VAC14 VAC14 component of PIKFYVE ... XM_017023441.2 79.1% 68.1% (many diffs)
7 human 55697 VAC14 VAC14 component of PIKFYVE ... XM_011523225.3 74.5% 70.9% (many diffs)
8 human 55697 VAC14 VAC14 component of PIKFYVE ... XM_017023442.2 73.6% 71.5% (many diffs)
9 human 55697 VAC14 VAC14 component of PIKFYVE ... NM_001351157.2 70% 70% 0_1ins702
10 mouse 234729 Vac14 Vac14 homolog (S. cerevisiae) NM_146216.2 89.3% 93.9% (many diffs)
11 mouse 234729 Vac14 Vac14 homolog (S. cerevisiae) XM_006530918.2 77.4% 81.4% (many diffs)
12 mouse 234729 Vac14 Vac14 homolog (S. cerevisiae) XM_006530919.3 63.7% 66.1% (many diffs)
13 mouse 234729 Vac14 Vac14 homolog (S. cerevisiae) XR_001778432.1 63.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2415
ORF length:
2346
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaaccccgag aaggatttcg cgccgctcac gcctaacatc gtgcgcgccc
121tcaatgacaa gctgtacgaa aagcggaagg tggcagcgct ggagatcgag aagctggtcc
181gggagttcgt ggcccagaac aataccgtgc aaatcaagca tgtgatccag accctgtccc
241aggagtttgc cctgtctcag cacccccaca gccggaaagg gggcctcatc ggcctggccg
301cctgctccat cgcactgggc aaggactcag ggctctacct gaaggagctg atcgagccag
361tgctgacctg cttcaatgat gcagacagca ggctgcgcta ctatgcctgc gaggccctct
421acaacatcgt caaggtggcc cggggcgctg tgctgcccca cttcaacgtg ctctttgacg
481ggctgagcaa gctggcagcc gacccagacc ccaatgtgaa aagcggatct gagctcctag
541accgcctttt aaaggacatt gtgactgaga gcaacaagtt tgacctggtg agcttcatcc
601ccttgttgcg agagaggatt tactccaaca accagtatgc ccggcagttc atcatctcct
661ggatcctggt tctggagtcg gtgccagaca ttaacctgct ggattacctg ccggagatcc
721tggatggact cttccagatc ctgggtgaca atggcaaaga gattcgcaaa atgtgtgagg
781ttgttcttgg agaattctta aaagaaatta agaagaaccc ctccagtgtg aagtttgctg
841agatggccaa catcctggtg atccactgcc agacaacaga tgacctcatc cagctgacag
901ccatgtgctg gatgcgggag ttcatccagc tggcgggccg cgtcatgctg ccttactcct
961ccgggatcct gactgctgtc ttgccctgct tggcctacga tgaccgcaag aaaagcatca
1021aagaagtggc caacgtgtgc aaccagagcc tgatgaagct ggtcaccccc gaggacgacg
1081agctggatga gctgagacct gggcagaggc aggcagagcc cacccctgac gatgccctgc
1141caaagcagga gggcacagcc agtggaggtc cagatggttc ctgtgactcc agcttcagta
1201gcggcatcag tgtcttcact gcagccagca ctgaaagagc cccagtgacc cttcacctcg
1261acgggatcgt gcaggtccta aactgccacc tcagtgacac ggccattggg atgatgacca
1321ggattgcagt tctcaagtgg ctctaccacc tctacatcaa aactcctcgg aagatgttcc
1381ggcacacgga cagcctcttt cccatcctac tgcagacgtt atcggatgaa tcggatgagg
1441tgatcctgaa ggacctggag gtgctggcag aaatcgcttc ctcccccgca ggccagacgg
1501atgacccagg ccccctcgat ggccctgacc tccaggccag ccactcagag ctccaggtgc
1561ccacccctgg cagagccggc ctactgaaca cctctggtac caaaggctta gaatgttctc
1621cttcaactcc caccatgaat tcttactttt ataagttcat gatcaacctt ctcaagagat
1681tcagcagcga acggaagctc ctggaggtca gaggcccttt catcatcagg cagctgtgcc
1741tcctgctgaa tgcggagaac atcttccact caatggcaga catcctgctg cgggaggagg
1801acctcaagtt cgcctcgacc atggtccacg ccctcaacac catcctgctg acctccacag
1861agctcttcca gctaaggaac cagctgaagg acctgaagac cctggagagc cagaacctgt
1921tctgctgcct gtaccgctcc tggtgccaca acccagtcac cacggtgtcc ctctgcttcc
1981tcacccagaa ctaccggcac gcctatgacc tcatccagaa gtttggggac ctggaggtca
2041ccgtggactt cctcgcagag gtggacaagc tggtgcagct gattgagtgc cccatcttca
2101catatctgcg cctgcagctg ctggacgtga agaacaaccc ctacctgatc aaggccctct
2161acggcctgct catgctcctg ccgcagagca gcgccttcca gctgctctcg caccggctcc
2221agtgcgtgcc caaccctgag ctgctgcaga ccgaagacag tctaaaggca gcccccaagt
2281cccagaaagc tgactcccct agcatcgact acgcagagct gctgcagcac tttgagaagg
2341tccagaacaa gcacctggaa gtgcggcacc agcggagcgg gcgtggggac cacctggacc
2401ggagggttgt cctcttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc
2461ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2521cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)